黄秋香,胡清华,王宏青.基于分子内电荷转移的近红外荧光探针的设计合成及其对N2H4识别和细胞成像性能研究[J].南华大学学报(自然科学版),2020,34(2):82~89, 96.[HUANG Qiuxiang,HU Qinghua,WANG Hongqing.Design and Synthesis of Near-infrared Fluorescence Probe Based on Intramolecular Charge Transfer Mechanism for N2H4 Identification and Cell Imaging[J].Journal of University of South China(Science and Technology),2020,34(2):82~89, 96.]
基于分子内电荷转移的近红外荧光探针的设计合成及其对N2H4识别和细胞成像性能研究
Design and Synthesis of Near-infrared Fluorescence Probe Based on Intramolecular Charge Transfer Mechanism for N2H4 Identification and Cell Imaging
投稿时间:2019-12-06  
DOI:
中文关键词:    异佛尔酮衍生物  近红外荧光探针  细胞成像
英文关键词:hydrazine  isophorone derivative  near-infrared fluorescent probe  cell imaging
基金项目:湖南省研究生科研创新项目(CX20190728)
作者单位E-mail
黄秋香 南华大学 化学化工学院,湖南 衡阳 421001 1942965927@qq.com 
胡清华 南华大学 化学化工学院,湖南 衡阳 421001  
王宏青 南华大学 化学化工学院,湖南 衡阳 421001  
摘要点击次数: 668
全文下载次数: 336
中文摘要:
      设计并合成了一种新型近红外异佛尔酮衍生物荧光探针(IS—NR—NH),利用肼(N2H4)特异性诱导的脱乙酰基反应,使得探针分子内电荷转移效应由弱变强,从而导致荧光增强,最终实现肼的选择性检测。该探针在HCO3-,SO42-,Cl-,SO32-,CO32-,Ac-,Br-,NO3-,NO2-,HSO3-,I-,F-,Mg2+,Co2+,Cd2+,Zn2+,Pb2+,Cr3+,Fe3+,Al3+,Mn2+,Ca2+,Ba2+等不同干扰物质存在下,仍能实现对N2H4的特异性比色及荧光识别。同时,在pH 7.4,二甲基亚砜与磷酸缓冲溶液的体积比为6∶4的体系中,探针IS—NR—NH对N2H4在0.02×10-3~0.4×10-3 mol/L的浓度范围内呈现出良好的线性关系,且检测限低至2×10-7 mol/L。此外,探针成功地实现了对HeLa细胞中N2H4的近红外荧光成像。
英文摘要:
      A novel near-infrare disophorone-derivative based fluorescent probe(IS—NR—NH) was designed and synthesized,and the utilization of N2H4-induced the acetylation deprotection reaction made the intramolecular charge transfereffect turned on and eventually realized the selective detection of hydrazine.The probe can still achieve specific colorimetric and fluorescent recognition of N2H4 even in the presence of different analytes such as HCO3-,SO42-,Cl-,SO32-,CO32-,Ac-,Br-,NO3-,NO2-,HSO3-,I-,F-,Mg2+,Co2+,Cd2+,Zn2+,Pb2+,Cr3+,Fe3+,Al3+,Mn2+,Ca2+,Ba2+.At the same time,in the system of pH 7.4 and the volume ratio of 6∶4 between dimethyl sulfoxide and phosphate buffer solution,the probe IS—NR—NH showed a good linear relationship with the concentration of N2H4 in the range of 0.02×10-3-0.4×10-3 mol/L,and the detection limit was as low as 2×10-7 mol/L.In addition,the probe successfully achieved the near-infrared fluorescence imaging of N2H4 in HeLa cells.
查看全文  查看/发表评论  下载PDF阅读器
关闭