| 徐小娜,蒋军辉,于军晖,吕昌银,胡青芳.枳实总黄酮的提取及含量测定[J].南华大学学报(自然科学版),2016,30(2):93~97.[XU Xiao-na,JIANG Jun-hui,YU Jun-hui,LV Chang-yin,HU Qing-fang.Extraction of the Total Flavonoids of Fructus Aurantii Immaturus and its Content Analysis[J].Journal of University of South China(Science and Technology),2016,30(2):93~97.] |
| 枳实总黄酮的提取及含量测定 |
| Extraction of the Total Flavonoids of Fructus Aurantii Immaturus and its Content Analysis |
| 投稿时间：2015-12-04 |
| DOI： |
| 中文关键词: 枳实 总黄酮 紫外分光光度法 超声波辅助提取法 |
| 英文关键词:Fructus Aurantii Immaturus total flavonoids UV spectrophotometric method ultrasonic assisted extraction method |
| 基金项目:国家自然科学基金资助项目(81473021);湖南省教育科学研究基金资助项目(14A123;12C0334) |
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| 中文摘要: |
| 利用超声波辅助法提取江枳实中的总黄酮,在单因素试验的基础采用正交试验获取最优提取工艺条件.以橙皮苷为标准品,在352 nm检测波长下,首次应用NaNO2-Al(NO3)3-NaOH体系作显色剂,对显色剂用量、显色时间和显色时的温度进行优化,采用紫外分光光度法在优化好的条件下测得江枳实总黄酮含量为6.29 mg/g.所得结果可为枳实黄酮类成分的深入研究和开发利用提供参考. |
| 英文摘要: |
| The total flavonoids of Fructus Aurantii Immaturus(FAI)from Jiangxi province were extracted by ultrasonic assisted extraction method.The optimal extracting conditions were obtained by orthogonal test design based on single factor experiment.With Hesperidin as reference standard and 352 nm as the detection wavelength,the NaNO2-Al(NO3)3-NaOH solution system was first employed for color reagent in determination of the total flavonoids of FAI by UV spectrophotometric method.Meanwhile,the dosage of color reagents,coloration time and coloration temperature were optimized.At last,under the optimum conditions mentioned above,the content of total flavonoids of FAI was detected as 6.29 mg/g.The results achieved will do good to the further research of the total flavonoids of FAI and medicinal materials’ exploitation. |
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