| 何逸飞,王奇金.Wnt/β-catenin信号通路相关蛋白的检测对甲状腺腺瘤患者及临床意义.[J].中南医学科学杂志.,2018,(4):417-419. |
| Wnt/β-catenin信号通路相关蛋白的检测对甲状腺腺瘤患者及临床意义 |
| Detection and clinical significance of Wnt/ beta -catenin signaling related proteins in patients with thyroid adenoma |
| 投稿时间：2017-12-27 修订日期：2018-06-07 |
| DOI：10.15972/j.cnki.43-1509/r.2018.04.023 |
| 中文关键词: 甲状腺腺瘤 Wnt/β-catenin 信号通路 Wnt-1蛋白 β-catenin蛋白 |
| 英文关键词:thyroid cancer Wnt/β-catenin signal pathway Wnt-1 protein β-catenin protein |
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| 中文摘要: |
| 本研究旨在探讨甲状腺腺瘤患者Wnt/β-catenin信号通路相关蛋白Wnt-1,β-catenin的检测及临床意义。采用RT-PCR方法检测Wnt-1,β-catenin mRNA表达情况,采用Western blot和免疫组化方法检测Wnt-1,β-catenin蛋白表达情况。结果显示,与对照组相比,疾病组Wnt-1,β-catenin mRNA和蛋白的表达量均升高。肿瘤组织中,疾病组的Wnt-1蛋白表达阳性率和β-catenin蛋白表达阳性率均高于对照组。结果表明,Wnt-1表达与甲状腺腺瘤患者肿瘤直径密切相关。β-catenin表达也与甲状腺腺瘤患者肿瘤直径密切密切相关。甲状腺腺瘤患者Wnt/β-catenin信号通路相关蛋白Wnt-1,β-catenin蛋白表达上调,且其表达与肿瘤生物学状态密切相关。 |
| 英文摘要: |
| The aim of this study was to evaluate the expressions of Wnt/β-catenin signal pathway related protein in thyroid cancer and their clinical significance. Quantitative real-time PCR was performed to assess mRNA levels of Wnt-1, β-catenin. Western blot assays and immunohistochemistry were performed to assess protein levels of Wnt-1, β-catenin. The results showed that, compared with the control group, the expression of Wnt-1, beta -catenin mRNA and protein increased in the disease group. In the tumor tissue, the positive rate of Wnt-1 protein expression and the positive rate of the expression of beta -catenin protein in the disease group were higher than those in the control group. The results showed that the expression of Wnt-1 was closely related to tumor diameter in patients with thyroid adenoma. The expression of beta -catenin is also closely related to tumor diameter in patients with thyroid adenoma. The expressions of Wnt/β-catenin signaling pathway related proteins Wnt-1, β-catenin are increased in patients with thyroid cancer, which may play an important role in the occurrence and development of thyroid cancer. |
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