| 温展翀,陈勍,黄妙玲,巴音吉力,徐干如,田春芳.CXC类趋化因子受体4基因多态性对新辅助化疗宫颈癌患者疗效及预后的影响.[J].中南医学科学杂志.,2016,(3):241-246, 250. |
| CXC类趋化因子受体4基因多态性对新辅助化疗宫颈癌患者疗效及预后的影响 |
| CXCR-4 gene Polymorphism and the Response in Cervical Cancer Patients Treated with Neoadjuvant Chemotherapy Major |
| 投稿时间：2016-04-18 修订日期：2016-05-15 |
| DOI： |
| 中文关键词: 趋化因子受体4 新辅助化疗 宫颈癌 生存预后 化疗反应 |
| 英文关键词:CXCR-4 gene Neoadjuvant chemotherapy Cervical cancer Prognosis Response to chemotherapy |
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| 中文摘要: |
| 目的 探讨CXCR4基因多态性对新辅助化疗宫颈癌患者化疗疗效的影响以及与新辅助化疗加广泛子宫切除的宫颈癌患者的预后关系。方法选取妇科肿瘤专科收治的62例IB2-IIB期宫颈癌患者；按照试剂盒说明书的指导蜡块组织提取组织DNA；PCR扩增基因组DNA片段；直接测序法检测DNA片段序列,测序结果采用图像分析软件“Chromas2”进行终点分析。结果扩增PCR产物为236 bp；62例样本中,CC基因型25例(40.3%),CT基因型37例(59.7%),TT基因型0例(0.0%)。T等位基因频率为2.98%；CXCR4基因Ile138Ile位点没有发现突变纯合子；位点Ile138Ile在病人中的基因分布频率与化疗反应无显著的相关性(P=0.771)；Ile138Ile位点CC型和CT型分布情况在宫颈浸润深度两组中(P=0.025)、淋巴结转移情况两组中(P=0.031)差异有统计学意义,而在化疗效果显示,患者年龄、组织类型、FIGO分期、肿瘤分化程度、肿瘤大小、脉管浸润、贫血情况各组中差异无统计学意义(P均＞0.05)；CXCR4基因ATT/ATC (Ile138Ile)位点CC型和CT型两组患者,三年生存率分别为86.4%、70.6%。Log-rank检验结果显示这两个年龄组患者的无病生存率和总生存率差别均无统计学意义。结论 CXCR4基因单核苷酸多态性位点Ile138Ile对宫颈癌的进展有影响； CXCR4基因单核苷酸多态性与新辅助化疗宫颈癌患者的生存预后可能无关。 |
| 英文摘要: |
| Objective To explore the influence of CXCR-4 gene polymorphism on the chemotherapeutic response and prognosis of cervical cancer patients who received NACT followed radical surgery.Methods 62 patients were enrolled in this study；Samples were recruited from the formalin-fixed,paraffin-embedded surgical specimens.DNA was extracted using the QIAamp DNA FFPE Tissue Kit；PCR of paraffin block genomic DNA；Direct sequence of DNA:All PCR products were further purified for commercial sequencing.ResultsThe length of the amplicon is 236bp； The CXCR4 genotype distributions were 25 CC (40.3%),37CT (59.7%) and 0 TT (0.0%)；Most clinicopathologic results failed to show a significant relationship with polymorphism except tumor grade.；The association CXCR4 gene polymorphism and response to chemotherapy:A statistically significant association was not found and favorable treatment with platinum based chemotherapy；The influence of CXCR4 gene polymorphism on survival:CXCR4 polymorphisms were not related to DFS or OS(P＞0.05).ConclusionThere are associations with CXCR4 polymorphisms and the prognosis of cervical cancer patients;.CXCR4 polymorphism has no significant association with the chemotherapeutic response of cervical cancer patients who received with platinum based neoadjuvant chemotherapy. |
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