| 阳志勇 ，尹文君 ，谢海涛 ，张秋桂.外排泵adeB基因与鲍曼不动杆菌多重耐药的关系.[J].中南医学科学杂志.,2015,43(3):266-268. |
| 外排泵adeB基因与鲍曼不动杆菌多重耐药的关系 |
| Relationship of Multidrug Resistance with Active Efflux Gene adeB in Clinical Isolates of Acinetobacter Baumannii |
| 投稿时间：2014-05-07 |
| DOI： |
| 中文关键词: 鲍曼不动杆菌 多重耐药 adeB |
| 英文关键词:Acinetobacter baumannii multiple resistance adeB |
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| 中文摘要: |
| 目的 探讨临床分离鲍曼不动杆菌主动外排基因adeB的表达与其多重耐药的关系。 方法 对临床分离的64株鲍曼不动杆菌采用纸片扩散法检测对抗菌药物的敏感性；罗丹明6G检测外排活性，RT-PCR检测外排泵基因adeB的分布和表达水平。 结果 64株鲍曼不动杆菌耐0～2种抗菌药物4株，耐3～5种抗菌药物33株，耐6～8种抗菌药物27株；罗丹明6G外排实验显示，外排增强的多重耐药菌株其外排基因adeB的表达明显增高。 结论 药物主动外排增强与鲍曼不动杆菌多重耐药相关；外排基因的过度表达可能是导致鲍曼不动杆菌多重耐药的主要原因。 |
| 英文摘要: |
| Objective To explore the relationship of multidrug resistance with abeB gene in the clinical isolates of Acinetobacter baumannii. Methods The agar doubling dilution method was used to identify the minimal inhibitory concentration（MIC） of 8 antibiotics for Acinetobacter baumannii.The efflux of Rhodamine 6G in 64 strains of clinical Acinetobacter baumannii was tested and the Reverse transcriptase polymerase chain reaction （RT-PCR） was used to detect the mRNA expression levels of active efflux gene adeB. Results Among 64 strains of clinical Acinetobacter baumannii 4 strains was resistant to 0～2 antibiotics,33 strains was resistant to 3～5 antibiotics,27 strains was resistant to 6～8 antibiotics.The expression levels of efflux gene adeB in the multiple drug-resistant strains which enhanced the efflux were also increased. Conclusions The effection of active efflux system of adeB may be one of the important multidrug resistance mechanisms of Acinetobacter baumannii. |
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