| 祖旭宇，谭莉，周茜，王艺蓁.Pokemon对乳腺癌MCF-7细胞侵袭迁移能力的影响.[J].中南医学科学杂志.,2014,42(6):545-548. |
| Pokemon对乳腺癌MCF-7细胞侵袭迁移能力的影响 |
| The Effect of Pokemon on the Invasion andMigration Ability of MCF-7 Cells |
| 投稿时间：2014-05-29 |
| DOI： |
| 中文关键词: pokemon 慢病毒载体 MCF-7细胞 细胞侵袭能力 |
| 英文关键词:pokemon lentiviral vector MCF-7cell line cell invasion ability |
| 基金项目:国家自然科学基金项目（81272355）和湖南省教育厅青年项目资助（12B108）. |
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| 中文摘要: |
| 目的建立稳定表达pokemon乳腺癌细胞系，并观察pokemon对乳腺癌细胞系MCF-7侵袭转移能力的影响。方法构建表达pokemon慢病毒载体，将Pokemon表达慢病毒载体转染MCF-7细胞,通过Western blot方法检测稳定性表达pokemon MCF-7细胞中Pokemon-GFP融合蛋白的表达情况，应用Transwell试验及划痕实验观察pokemon的高表达对 MCF-7细胞侵袭迁移能力的影响。结果成功建立稳定表达pokemon的MCF-7细胞系，pokemon可抑制MCF-7细胞侵袭能力。 结论Pokemon可抑制MCF-7细胞侵袭能力。 |
| 英文摘要: |
| ObjectiveTo establish MCF-7 cells with stablely ectopic pokemon expression and to assess the effect of pokemon on the invasion and migration of MCF-7 cells.MethodsImmunofluorescence and Western blot were used to screen the MCF-7 cell clones with ectopic pokemon expression.Transwell and wound healing analysis were utilized to determine the effect of pokemon on the invasion and migration of MCF-7 cells.ResultsThe MCF-7 cell line with stablely ectopic pokemon expression was established and Pokemon was demonstrated to suppress the invasion ability of MCF-7 cells. |
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