| 刘斌，刘婧，刘卓然，汪翼，吴广.纺锤体检查点蛋白Cenp-E在肝癌组织的表达及临床意义.[J].中南医学科学杂志.,2014,42(5):459-461. |
| 纺锤体检查点蛋白Cenp-E在肝癌组织的表达及临床意义 |
| Expression of the Spindle Checkpoint Protein Cenp-E in the Human Hepatom Tissue and Its Clinical Significance |
| 投稿时间：2014-01-17 |
| DOI： |
| 中文关键词: 纺锤体检查点 Cenp-E基因 肝癌 |
| 英文关键词:spindle checkpoint Cenp-E gene hepatoma |
| 基金项目:湖南省教育厅课题（No.13C796）. |
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| 中文摘要: |
| 目的观察纺锤体检查点蛋白（Cenp-E）在肝癌组织和人类正常肝组织表达水平的差异，以探讨Cenp-E蛋白在肝癌细胞中的表达及临床意义。方法用荧光定量PCR和Western blot分别检测Cenp-E基因和蛋白在肝癌组织和正常肝组织的表达水平。结果正常肝组织中Cenp-E基因mRNA水平(0.023 78±0.009 73)明显高于肝癌组织（0.014 58±0.008 73）；Cenp-E蛋白表达量在正常肝组织中（0.656±0.012）明显高于在肝癌组织中的表达（0.301±0.009），且二者有显著性差异。结论Cenp-E低表达在肝癌的发生过程中起重要作用。 |
| 英文摘要: |
| ObjectiveTo study the differential expression and its clinical significance of the spindle checkpoint protein Cenp-E gene between the human hepatoma and hepatic tissue.MethodsReal-time fluorescent-quantitation PCR and Western blot were employed to detect the expressions of the Cenp-E mRNA and protein,respectively.ResultsThe expression of the Cenp-E mRNA in human hepatic tissue(0.023 78±0.009 73) was higher than that in human hepatoma tissue（0.014 58±0.008 73）.The expression of the Cenp-E protein in human hepatic tissue（0.656±0.012） was higher than that in human hepatoma tissue（0.301±0.009）.ConclusionCenp-E gene low expression in human hepatocellular carcinoma plays an important role in the its developmental process. |
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