| 周昌宁，曹海宁.LTA对BALB/c幼鼠肠黏膜TLR4表达的影响.[J].中南医学科学杂志.,2014,42(5):455-458. |
| LTA对BALB/c幼鼠肠黏膜TLR4表达的影响 |
| Changes of Expression of TLR4 Induced by LTA in Intestinal Mucosa of BALB/c Mice Pups |
| 投稿时间：2014-05-30 |
| DOI： |
| 中文关键词: 脂磷壁酸 肠黏膜 二甲基亚砜 TLR4 |
| 英文关键词:Lipoteichoic acid intestinal mucosa Dimethyl sulfoxide TLR4 |
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| 中文摘要: |
| 目的探讨脂磷壁酸（LTA）对BALB/c幼鼠肠黏膜Toll样受体4（TLR4）表达的影响。方法将30只1周龄BALB/c幼鼠随机分为正常组、溶媒组和LTA组，分别给予NS、DMSO和LTA各0.1 mL一次性灌胃处理，并于处理后第1周、第2周和第4周分别取幼鼠回肠末端、回盲部及结肠组织，HE染色观察各段组织病理学变化，免疫组化检测TLR4蛋白表达情况。结果HE染色显示与正常组相比，溶媒组和LTA组幼鼠肠黏膜组织均有炎性浸润尤以经LTA处理后第1周的回肠末端最明显：其黏膜结构完整性受损，黏膜间隙增宽，炎性细胞浸润，大量细胞气球样变性甚至坏死。免疫组化结果表明各组不同组织不同时间段均表达TLR4;LTA组表达量最高，与正常组和溶媒组相比差异均有显著性（P＜0.05）；各组同一组织TLR4表达随时间延迟表达量增多以第4周最高，与第1周和第2周相比差异有显著性（P＜0.05）；相同组织在不同组别以结肠表达最高并随时间延长而增加（P＜0.05）；第4周LTA组结肠组织表达量最高，差异均有显著性（P＜0.05）。结论LTA能上调肠黏膜TLR4表达并随着时间延长而增加，其对LTA识别和反应部位可能主要位于结肠。 |
| 英文摘要: |
| ObjectiveTo observe the expression of TLR4 in intestinal mucosa of BALB/c mice pups.Method30 BALB/c mice postnatal one week were randomly divided into normal group,solvent group and LTA group,and were administrated with NS,DMSO or LTA 0.1ml one time,respectively.The pups intestine were taken out at the first,second and fourth week and the terminal ileum,cecum and colon were separated.The pathological change of the tissues were analyzed by HE staining and the expression of the TLR4 were detected by immunohistochemistry (IHC).ResultHistological examination showed that inflammatory infiltration was observed in solvent control group and LTA group.The integrity of mucosal structure was injured,the gap of the mucosa was widened and inflammatory cell infiltration or ballooning degeneration even necrosis was observed,especially in the terminal ileum of the LTA group at first week.The results of the immunohistochemistry indicated that the expression of TLR4 were detected in different tissues at different time period in three groups; the expression level of TLR4 in LTA group was the highest,compared with normal group and solvent group (P＜0.05); The expression of TLR4 in the same tissues of different groups were increased over time and the level of TLR4 at the fourth week were higher when compared with which at the first or second week(P＜0.05); the expression of TLR4 in colon were the highest and increased over time and the level of TLR4 in colon at the 4th week was the highest(P＜0.05).ConclusionLTA can up-regulate the expression of TLR4 in the intestinal mucosa,and the site of the LTA recognition and reaction may be mainly located in the colon. |
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