| 万青松，谢红萍，杨波.维拉帕米阻抑UUO大鼠肾间质纤维化.[J].中南医学科学杂志.,2014,42(5):440-442. |
| 维拉帕米阻抑UUO大鼠肾间质纤维化 |
| Anti-fibrosis Effect of Varapamil on Rat Kidney with Unilateral Ureteral Obstruction |
| 投稿时间：2013-12-18 |
| DOI： |
| 中文关键词: 肾间质纤维化 氧化应激 内皮素-1 维拉帕米 |
| 英文关键词:renal interstitial fibrosis oxidative stress endothelin-1 varapamil |
| 基金项目:衡阳市科技局课题 (2009 KJ39). |
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| 中文摘要: |
| 目的探讨维拉帕米对单侧输尿管梗阻（UUO）大鼠肾间质纤维化的影响。方法将24只大鼠随机分为对照组（即假手术组）、UUO模型组、维拉帕米治疗组，采用单侧输尿管梗阻模型，术后8天观察梗阻肾组织病理改变，比色法测定肾组织羟脯氨酸（HYP）、丙二醛（MDA）和超氧化物歧化酶（SOD）含量,并应用放射免疫法检测血浆和肾组织内皮素-1（ET-1）的含量。结果与对照组比较，模型组肾组织HYP和MDA含量明显升高，血浆和肾组织内皮素-1含量明显升高，肾组织SOD含量显著下降（P<0.01）,维拉帕米治疗组与模型组相比，肾组织HYP和MDA含量明显下降，血浆和肾组织内皮素-1下降，肾组织SOD含量显著升高，差异均有显著性（P<0.05）。结论维拉帕米能减少单侧输尿管梗阻大鼠脂质过氧化物的产生，降低血浆和肾组织内皮素-1的含量，增加抗氧化酶的含量，从而阻抑UUO大鼠肾间质纤维化。 |
| 英文摘要: |
| ObjectiveTo investigate the effect of varapamil on kidney of rats with interstitial fibrosis after unilateal ureteral obstruction(UUO).MethodsTwenty-four rats were randomLy assigned to shame operation group(normal group),UUO group and varapamil treatment group after unilateral ureteral obstruction.Renal tissues were examined by light microscopy 8 days after operation.Hyd -roxyl puamino acid (HYP)，Malondiadehyde(MDA) and superoxide dismutase(SOD) content were measured.Endothelin (ET-1) was measured by radioimmunoassay.ResultsIn comparison with shame operation group,HYP and MDA content significantly increased,the level of plasmic and renal endothelin-1 significantly increased,SOD content significantly decreased（P<0.01）.In comparison with UUO group,varapamil signicantly ameliorated what had mentioned (P<0.05).ConclusionsVarapamil can suppress renal interstitial fibrosis by reducing lipid peroxidation production and reducing the level of plasmic and renal endothelin-1 and improving antioxidative enzyme contents. |
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