| 陈健文，张文平，周长华，黄守坚，陈少锐.草麻黄抗补体组份对血小板变形及内皮细胞损伤作用.[J].中南医学科学杂志.,2014,42(5):436-439. |
| 草麻黄抗补体组份对血小板变形及内皮细胞损伤作用 |
| Isolation of Anti-complement Components from Ephedra Sinica andits Protective Effect on Complement-dependent Injury |
| 投稿时间：2014-03-18 |
| DOI： |
| 中文关键词: 草麻黄 补体 攻膜复合物 补体依赖性损伤 |
| 英文关键词:ephedra sinica complement member attack complex complement-dependent injury |
| 基金项目: |
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| 中文摘要: |
| 目的从草麻黄中分离抗补体组份并观察其抗补体依赖性的损伤作用。方法采用柱层析结合紫外吸收观察的方法分离抗补体组份；采用比浊法观察组份对眼镜蛇毒因子引起的血小板变形的影响；采用MTT法观察组份对异种血清对内皮细胞损伤的影响。结果紫外吸收为196 nm的组份具有抗补体作用，麻黄组份能有效抑制眼镜蛇毒因子引起的血小板变形，高、中、低剂量对血小板变形的抑制率分别为49.8%±10.7%、32.8%±4.2%、22.5%±6.2%；麻黄组份能有效减轻异种血清对内皮细胞的损伤，高、中、低剂量对补体致内皮细胞损伤抑制率分别为88.1%±7.1%、79.8%±3.3%、65.0%±4.5%。结论麻黄抗补体组份对补体依赖性损伤有保护作用。 |
| 英文摘要: |
| ObjectiveTo isolate the anti-complement components from ephedra sinica and investigate its protective effect on complement-dependent injury.MethodsAnti-complement components were seprerated by using column chromatography;turbidimetric assay was used to investigate the effect of anti-complement component on CVF-induced platelet metamorphose;MTT method was used to investigate the effect of anti-complement components on endothelial cells injury caused by rabbit serum.ResultsAnti-complement components effectively reduced CVF-induced platelet metamorphose and attenuated the endothelial cells injury caused by rabbit serum.ConclusionAnti-complement components may have protective effect on complement-dependent injury. |
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