| 易亚乔，肖碧跃，刘英飞，刘林，陈懿，廖君，葛金文.加味脑泰方对血管性痴呆大鼠学习记忆和海马区NR2A及NR2B表达的影响.[J].中南医学科学杂志.,2014,42(1):17-20. |
| 加味脑泰方对血管性痴呆大鼠学习记忆和海马区NR2A及NR2B表达的影响 |
| Effect of the Supplemented Naotaifang on the Vascular DementiaRats Learning and Memory and the Expression of the NR2A,NR2B in Hippocampus |
| 投稿时间：2013-09-12 |
| DOI： |
| 中文关键词: 加味脑泰方 血管性痴呆 海马 |
| 英文关键词:Supplemented Naotaifang Vascular dementia hippocampus |
| 基金项目:湖南省中医药科研基金重点项目(201213)；湖南省教育厅青年基金(13B083)；湖南省教育厅科学研究一般项目(13C958)；湖南省科技厅项目(2013TT2025). |
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| 中文摘要: |
| 目的观察加味脑泰方对血管性痴呆(VaD)大鼠学习记忆的影响，同时检测海马区NR2A及NR2B表达情况以探讨其可能机制。方法将36只SD大鼠随机均分成假手术组、模型组和加味脑泰方组。采用四血管阻断法制备血管性痴呆模型。Morris水迷宫评价大鼠学习记忆能力；应用免疫组织化学技术观察各组大鼠海马NR2A及NR2B的表达情况。结果加味脑泰方组逃避潜伏期(27.4±6.5 s)显著低于模型组(64.4±10.1 s)，穿越平台次数（8.1±0.98次）显著多于模型组（5.6±0.8次），差异均有显著性(P<0.05)；加味脑泰方组海马NR2A+、NR2B+分别是22.4±2.6、18.9±3.0个，模型组分别为14.8±3.3、12.6±2.9个，两组NR2A+、NR2B+相比，差异均有显著性(P<0.05)；加味脑泰方组海马NR2A+、NR2B+的平均光密度值较模型组显著提高(P<0.05)。结论加味脑泰方可改善VaD大鼠学习记忆能力，其机制可能与上调大鼠海马区NR2A及NR2B的表达相关。 |
| 英文摘要: |
| ObjectiveTo observe the effect of the supplemented Naotaifang on the Vascular dementia rats learning and memory and the expression of the NR2A,NR2B in Hippocampus.Methods36 rats were randomly divided into three groups,the dementia model was made using 4-vessel occlusion,the learning and memory was evaluated by Morris water maze;the expression of NR2A,NR2B was watched by the immunohistochemical technique.ResultsThe escape latency in the SNTF group is (27.4±6.5)s,which is lower than that(64.4±10.1)s in the vehicle group(P<0.05);the times across platform between the SNTF group and vehicle group is (8.1±0.98)s,(5.6±0.8)s respectively(P<0.05);the NR2A+,NR2B+ in the SNTF group is 22.4±2.6,18.9±3.0 respectively,the NR2A+,NR2B+ in the vehicle group is 14.8±3.3,12.6±2.9 respectively,which was statistical comparing the NR2A+,NR2B+ between two groups(P<0.05);the mean optical density of the NR2A+,NR2B+ in the SNTF group is higher than that in the vehicle group(P<0.05).ConclusionSupplemented Naotaifang can up-regulate the expression of NR2A,NR2B in hippocampus,and improve the vascular dementia rats learning and memory. |
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