| 唐三元，黄卫国，朱媛，苏菊，陈琳，殷耒兰，李跃华.鼻咽癌顺铂耐药细胞系的建立及其耐药性变化.[J].中南医学科学杂志.,2013,41(5):437-439. |
| 鼻咽癌顺铂耐药细胞系的建立及其耐药性变化 |
| Establishment of cis-Diammine-Dichloro- Platinum (cDDP) -resistant NPCCell Lines (CNE2/cDDP) and Study of the Changes of Drug Resistance |
| 投稿时间：2013-05-27 |
| DOI： |
| 中文关键词: 顺铂 鼻咽癌 多药耐药性 细胞系（CNE2/cDDP） |
| 英文关键词:cDDP NPC multi-drug resistance cell lines(CNE2/cDDP) |
| 基金项目:国家自然科学基金（81172210），湖南省自然科学衡阳联合基金（10JJ9017）. |
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| 中文摘要: |
| 目的通过顺铂（cDDP）浓度递增法建立鼻咽癌耐药的细胞株CNE2/cDDP，并研究其耐药性变化。 |
| 英文摘要: |
| ObjectiveTo study the changes of drug resistence of NPC by establishing the cis-Diammine-Dichloro- Platinum (cDDP) -resistant NPC cell lines (CNE2/cDDP) through cDDP concentration progressively increased method.MethodsCNE2/cDDP was established by cDDP concentration progressively increased method.Morphology study was performed by invert microscope,and drug resistance to cDDP,Vincristin,Carboplatin,Taxotere,and 5-fluorouracil were detected by MTT.ResultsMTT showed that there was significant increase of IC50 and resistance index in CNE2/cDDP compared to CNE2，after dealt with cDDP,Vincristin,Carboplatin,Taxotere,and 5-fluorouracil.And the drug resistance of cDDP in CNE2/cDDP was the highest,and RI (resistance index) was increased to 200.6-folds compared to CNE2.While the lowest drug resistance was in 5-Fu,and RI was increased to 2.8-folds compared to CNE2.There were clone aggregation and slow growth speed in CNE2/cDDP and more longer doubling time increased from 18 h in CNE2/cDDP to 24 h in CNE2.And there were much smaller volume in CNE2/cDDP compared with CNE2.ConclusionCNE2/cDDP may serve as an ideal cell line used for study of drug resistance in NPC for its multi-drug resistance. |
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