| 李招兵，马小峰，邓立普，罗刚.蝮蛇毒诱导小鼠心肌损伤与血清NT-proBNP表达的相关性.[J].中南医学科学杂志.,2013,41(4):357-360. |
| 蝮蛇毒诱导小鼠心肌损伤与血清NT-proBNP表达的相关性 |
| Correlation Between Myocardial Injury and Serum NT-proBNP on Mice Induced by Agkistrodon Halys Pallas Venom |
| 投稿时间：2012-10-30 |
| DOI： |
| 中文关键词: 蝮蛇毒 心肌损伤 N末端B型尿钠肽原 肌钙蛋白 |
| 英文关键词:agkistrodon snake venom myocardial injury N-terminal pro-brain natriuretic peptide cTnT |
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| 中文摘要: |
| 目的观察蝮蛇毒诱导的小鼠心肌损伤与血清NT-proBNP表达情况，以探讨蝮蛇毒诱导心肌损伤的机制。方法96只小鼠随机分成蝮蛇毒组和正常组，蝮蛇毒组1.50 mg/kg蝮蛇毒肌注，正常组生理盐水肌注。肌注后1 h、12 h、24 h、48 h时酶联免疫吸附测定（ELISA）检测血清肌钙蛋白（cTnT）和N末端B型尿钠肽原（NT-proBNP）水平，并观察心肌组织病变。对心肌病变积分、血清cTnT水平与血清NT-proBNP水平行直线相关分析。结果蝮蛇毒组各时相点心肌病变积分和血清NT-proBNP表达水平高于正常组(P＜0.05)，且随时间延长而增高(P＜0.05)；除1 h组外，蝮蛇毒组各时相点血清cTnT水平均高于正常组(P＜0.05)，且随时间延长而增高(P＜0.05)。小鼠心肌病变积分与血清NT-proBNP水平呈显著正相关(r＝0.647，P＜0.05)，血清cTnT水平与NT-proBNP水平呈显著正相关(r＝0.529，P＜0.05)。结论蝮蛇毒可致小鼠急性心肌损伤，随时间的延长心肌损伤加重，NT-proBNP水平增高。NT-proBNP的合成与释放增加可能是蝮蛇毒诱导心肌损伤的机制之一。 |
| 英文摘要: |
| ObjectiveObserve serum NT-proBNP levels to explore the mechanisms by Agkistrodon snake venom-mediated myocardial injury on mice.Methods The 96 mice were randomly divided into experimental and control groups. Experimental group was delivered by intramuscular injection of 1.50 mg/kg and control group was delivered by intramuscular injection of saline. Serum cTnT and NT-proBNP were detected by ELISA and pathological changes of myocardial were observed after 1 h, 12 h, 24 h, 48 h. Linear correlation analysis were performed in myocardial histopathologic scores, serum cTnT and NT-proBNP levels. ResultsMyocardial histopathologic scores and serum NT-proBNP levels at each time point in experimental group were higher than control group(P＜0.05), and increased with time(P＜0.05); Besides 1 h group, serum cTnT levels at each time point in experimental group were higher than control group(P＜0.05), and increased with time(P＜0.05); myocardial histopathologic scores was significantly and positively correlated with serum NT-proBNP levels (r＝0.647, P＜0.05); serum cTnT levels was significantly and positively correlated with serum NT-proBNP levels (r＝0.529, P＜0.05).ConclusionAgkistrodon snake venom can lead to acute myocardial injury of mice. With the extension of time, the more severe myocardial injury, the higher serum NT-proBNP levels increased significantly. Over-expression of NT-proBNP may be one of the mechanisms that agkistrodon snake venom-mediated myocardial injury. |
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