| 李湘宏，余晓玲，姜莉云.HPLC法测定胃血止糊剂中大黄素和大黄酚的含量.[J].中南医学科学杂志.,2012,40(6):616-620. |
| HPLC法测定胃血止糊剂中大黄素和大黄酚的含量 |
| Determination of the Archin and Chrysophanol in Bastric Blood Paste by HPLC |
| 投稿时间：2011-11-04 |
| DOI： |
| 中文关键词: 胃血止糊剂 高效液相色谱 大黄素 大黄酚 |
| 英文关键词:stomach blood paste HPLC archin chrysophanol |
| 基金项目:云南省卫生厅院内制剂专项资助项目—云财社［2005］198号. |
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| 中文摘要: |
| 目的通过测定胃血止糊剂中大黄素和大黄酚含量，为控制该制剂质量提供相关指标和方法。方法用高效液相色谱(HPLC)建立测定大黄中大黄素和大黄酚含量的方法。结果大黄素和大黄酚的平均回收率为97.56%，98.81%，变异系数（RSD）分别为1.27%,1.47%。大黄素的回归方程为y=2 842.3x-4.159 1(r=0.999 9)，线性范围为0.009 2~0.184 μg；大黄酚的回归方程为y=4 080x-5.985 3(r=0.999 8)，线性范围为0.020 8~0.416 μg。结论本文建立的HPLC法测定大黄素和大黄酚在胃血止糊剂中的含量，简便易行，重现性好，具有一定的专属性，可作为该药质量控制的指标和方法。 |
| 英文摘要: |
| ObjectiveTo determine the content of archin and chrysophanol in gastric blood paste,by which provide the indexes for evaluating the preparation quality control.MethodsTo establish the method of determination of archin and chrysophanol in rhubarb by high performance liquid chromatography (HPLC).ResultsThe average recovery of Emodin or Chrysophanol was 97.56%,98.81%,and the relative standard deviation (RSD) was 1.27%,1.47%,respectively.The regression equation of archin is y=2 842.3x-4.159 1 (r=0.999 9),the linear range is 0.009 2~0.184 μg.The regression equation of chrysophanol is y=4 080x-5.985 3(r=0.999 8),the linear range is 0.020 8~0.416 μg.ConclusionsThe HPLC method set for determination of Archin and Chrysophanol in Bastric Blood Paste in this study is Simple,good for repetition and Specialization,which could provide the indexes for evaluating the preparation quality control. |
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