| 付成效，李华文.HPLC法同时测定人血清中氯硝西泮、硝西泮、三唑仑浓度.[J].中南医学科学杂志.,2011,39(4):457-459. |
| HPLC法同时测定人血清中氯硝西泮、硝西泮、三唑仑浓度 |
| Simultaneous Determination of Natrazepam，Clonazepam，Triazolam in Human Serum Concentration by HPLC |
| 投稿时间：2010-09-07 |
| DOI： |
| 中文关键词: HPLC 硝西泮 氯硝西泮 三唑仑 |
| 英文关键词:HPLC carbamazepine phenytoin phenobarbital |
| 基金项目: |
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| 中文摘要: |
| 目的建立单步萃取-HPLC法同时测定人血清中硝西泮、氯硝西泮、三唑仑浓度。方法血清样品采用二氯甲烷单步萃取，采用Ultimate XB-C18色谱柱(250.0 mm×4.6 mm，5 μm)，流动相为甲醇∶水∶乙腈=10∶56∶34,流速为1 mL/min,检测波长为254 nm，柱温为30℃，卡马西平为内标物。结果硝西泮、氯硝西泮、三唑仑的线性范围分别为32.5～650.0 μg/L（r=0.9997）、31.5～630.0 μg/L（r=0.9999）、74～1 480 μg/L（r=0.9997），最低检测限分别为8.13 μg/L、7.88 μg/L、18.5 mg/L，日内精密度和日间精密度良好，回收率较高。结论本法操作简便，结果准确可靠。可满足临床快速监测的需要。 |
| 英文摘要: |
| ObjectiveTo establish a single step method for the determination of the concentration of the natrazepam(NZ)，clonazepam(CZ)，triazolam(TZ). MethodsThe serum sample was extracted once with Dichloromethane.The residue was dissolved and analyzed with Ultimate XB-C18(250.0 mm×4.6 mm，5 μm)，mobilephase was methanol∶water∶acetonitrile=10∶56∶34，low rate was 1.0 mL/min.The detective UV wavelength was 254 nm，the column temperature was 30℃，carbamazepine was used as internal standard. ResultsThe linear relation was in the range of 32.5～650.0 μg/L（r=0.999 7），31.5～630.0 μg/L（r=0.999 9）and 74～1 480 μg/L（r=0.999 7）.The lowest detectable concentration in plasma was 8.13 μg/L,7.88 μg/L，18.5 mg/L，the intra-day precision and inter-day precision were all good，the average recovery rate was comparatively high.ConclusionThe method is rapid,accurate and practical.It can be used as the fast method by TDM. |
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