| 陈东，付成效.RP-HPLC法测定人血清中茶碱浓度的改进.[J].中南医学科学杂志.,2011,39(1):111-113. |
| RP-HPLC法测定人血清中茶碱浓度的改进 |
| Determination of Theophylline Concentration in Human Serum by Improved RP-HPLC Method |
| 投稿时间：2009-09-10 |
| DOI： |
| 中文关键词: HPLC 茶碱 血清浓度 |
| 英文关键词:HPLC theophlline serum concentration |
| 基金项目: |
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| 中文摘要: |
| 目的建立反相高效液相色谱法测定人血清中茶碱浓度。方法血清样品加入咖啡因为内标，10%三氯醋酸去蛋白，采用Ultimate XB-C18(250 mm×4.6 mm，5 μm)色谱柱分离，流动相为甲醇∶水=30∶70,流速为1.0 mL/min,检测波长为273 nm，柱温为30℃。结果茶碱在1.76～44.00 mg/L浓度范围线性关系良好，r=0.999 6，血清最低检测限为0.44 mg/L，日内精密度和日间精密度良好，回收率较高。结论本法操作简便，结果准确可靠。可满足临床快速监测的需要。 |
| 英文摘要: |
| ObjectiveTo determine theophylline rapidly in serum by concentration of theophylline in serum.MethodThe separation was performed on Ultimate XB-C18(4.6 mm×250 mm,5 μm) column with methanol-water( 30∶70 ) as mobile phase.The flow rate was 1.0 mL/min.The wavelength for detection was 273 nm.The column temperature was 30℃.Trichloroacetic acid was used to precipitate the serum protein.ResultsThe calibration curve had good linearity between 1.76～44.00 mg/L,r=0.999 6.The intra-day precision and inter-day precision were all good.The average recovery were all respective.ConclusionsThis method is accuate,rapid,and simple.It can be used as a roution method by TDM. |
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