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| U(VI)还原菌的筛选及其抗铀胁迫的蛋白质组学研究 |
| Screening of U(VI)-Reducing Bacteria and Proteomic Study on Their Response to Uranium Stress |
| 投稿时间：2025-04-28 修订日期：2025-05-13 |
| DOI： |
| 中文关键词: U(VI)还原 生物修复 芽孢杆菌 蛋白组学 |
| 英文关键词:U(VI) reduction Bioremediation Bacillus sp. X02 Proteomics |
| 基金项目:湖南省自然科学基金资助项目(2022JJ30491)；湖南省教育厅重点项目(22A0308) |
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| 中文摘要: |
| 为获得铀污染生物修复的高效菌种资源，并探究其在U(VI)生物还原过程中的分子作用机制。本研究通过厌氧富集培养和PTYG平板分离，从中国南方某退役铀尾矿库沉积物中分离筛选了三株耐铀菌株（X01、X02、X03）。在厌氧条件下，X02对初始浓度100 mg/L U(VI)的去除率高达98.65%，显著高于X01（79.69%）和X03（82.71%）。基于16S rDNA序列的系统发育分析鉴定X02为芽孢杆菌属（Bacillus sp.），生长动力学分析显示，Bacillus sp. X02在12 h进入对数生长期，且此时代谢活性达到峰值。进一步采用iTRAQ蛋白质组学技术分析了Bacillus sp.X02在U(VI)胁迫下的差异表达蛋白，发现在M/N组间共有229个差异表达蛋白，其中90个上调，139个下调。KEGG通路分析表明，上调蛋白主要富集于糖代谢、氨基酸代谢及应激响应通路，而下调蛋白则涉及细胞运动和膜运输功能，这些结果表明Bacillus sp. X02通过调控代谢网络和生理功能来适应铀胁迫。 |
| 英文摘要: |
| To obtain efficient microbial resources for uranium bioremediation and elucidate the molecular mechanisms underlying U(VI) bioreduction, this study isolated and screened three uranium-tolerant strains (X01, X02, X03) from sediments of a decommissioned uranium tailings pond in southern China through anaerobic enrichment culture and PTYG plate separation. Under anaerobic conditions, X02 exhibited a U(VI) removal rate of 98.65% at an initial concentration of 100 mg/L, significantly higher than those of X01 (79.69%) and X03 (82.71%). Phylogenetic analysis based on 16S rDNA sequences identified X02 as Bacillus sp. Growth kinetic analysis revealed that Bacillus sp. X02 entered the logarithmic phase at 12 h, with metabolic activity peaking at this stage. Further iTRAQ-based proteomic analysis of Bacillus sp. X02 under U(VI) stress identified 229 differentially expressed proteins (90 upregulated, 139 downregulated) between the treatment (M) and control (N) groups. KEGG pathway analysis demonstrated that upregulated proteins were primarily enriched in carbohydrate metabolism, amino acid metabolism, and stress response pathways, whereas downregulated proteins were associated with cellular motility and membrane transport functions. These findings indicate that Bacillus sp. X02 adapts to uranium stress by reprogramming its metabolic network and physiological functions. |
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