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| 基于转录组测序分析拟南芥对铀胁迫的跨代响应 |
| Analysis of the Transgenerational Responses in Arabidopsis thaliana to Uranium Stress Based on Transcriptome Sequencing |
| 投稿时间：2025-03-20 修订日期：2025-04-01 |
| DOI： |
| 中文关键词: 铀 拟南芥 高通量测序 跨代效应 |
| 英文关键词:uranium arabidopsis thaliana high-throughput sequencing transgenerational effects |
| 基金项目:国家自然科学基金面上项目(12275123) |
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| 中文摘要: |
| 目前在植物修复铀污染土壤领域,关于植物对铀胁迫的跨代响应研究较少,这在一定程度上制约了植物修复技术的长效性评估与遗传改良策略制定。通过在富铀土壤中培养亲代拟南芥,探究其子代在解除铀胁迫后基因转录表达水平变化。实验结果发现，与对照组相比，低、高浓度铀胁迫组拟南芥子代分别有2 096和1 170个差异表达基因（differentially expressed genes，DEGs）。拟南芥子代的DEGs在分子功能、细胞组分和生物过程三部分中均存在显著富集，且普遍表现为上调基因数量大于下调基因数量。拟南芥子代的DEGs主要分为5个不同的KEGG子系统，两比较组的DEGs显著富集的KEGG通路均为8条。该研究揭示了铀胁迫对植物的跨代效应现象，为利用诱变育种和基因重组等技术筛选超富集植物品种奠定了基础。 |
| 英文摘要: |
| Current research on the transgenerational responses of plants to uranium (U) stress in the field of phytoremediation of U-contaminated soils remains limited, which constrains the long-term efficacy evaluation of phytoremediation technologies and the formulation of genetic improvement strategies. This study investigated the changes in transcriptional expression levels of progeny Arabidopsis thaliana after parental plants were cultivated in U-enriched soil followed by U stress removal. Experimental results revealed 2 096 and 1 170 differentially expressed genes (DEGs) in the progeny of low- and high-concentration U-stressed groups, respectively, compared to the control group. These DEGs exhibited significant enrichment across molecular functions, cellular components, and biological processes, with the number of upregulated genes generally exceeding downregulated genes. The DEGs were classified into five distinct KEGG subsystems, and both comparison groups showed significant enrichment in eight KEGG pathways. This study elucidates the transgenerational effects of U stress on plants, laying the foundation for screening hyperaccumulator varieties through mutagenesis breeding and genetic recombination technologies. |
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