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| 核酸适配体/G4-DNAzyme联用荧光探针用于Hg2+的高灵敏快速测定 |
| Aptamer/G4-DNAzyme-Based Fluorescent Probe for Highly Sensitive and Rapid Detection of Hg2+ |
| 投稿时间：2025-03-19 修订日期：2025-03-31 |
| DOI： |
| 中文关键词: 汞离子 G-四链体 核酸适配体 检测 |
| 英文关键词:mercury ion G-quadruplex aptamer detection |
| 基金项目:湖南省教育厅优秀青年项目 |
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| 中文摘要: |
| 重金属污染严重威胁公众健康。本研究开发了一种联合核酸适配体特异性识别与G-四链体/氯化血红素（G4-DNAzyme）催化放大的荧光传感方法，用于汞离子（Hg2+）的高灵敏快速检测。利用与胸腺嘧啶（T）的特异性结合，设计含T-Hg2?-T配位结构的双链探针。当Hg2+存在时触发探针构象转换，形成高活性G4-DNAzyme，催化邻苯二胺（OPD）氧化生成强荧光产物2,3-二氨基吩嗪（DAP），实现信号放大。在最佳条件下，可定量检测0.1 μg/L~1.2 μg/L范围内的Hg2+，检出限为0.1 μg/L。该方法在衡阳市湘江水样、南华大学自来水样与池塘水样品中同时具备良好的检出性能，加标回收率在93 %~107 %之间，相对标准偏差（RSDs）为0.58 %~8.33 %之间。本研究建立的检测体系兼具高灵敏度及特异性、低成本及实用性，能有效监测饮用水源中Hg2?污染水平，为预防汞暴露导致的神经毒性、肝肾损伤等公共卫生问题提供关键技术支撑。 |
| 英文摘要: |
| Heavy metal pollution poses a serious threat to public health. In this work, we have developed a new fluorescence biosensing platform for highly sensitive and rapid detection of mercury ions (Hg2+), integrating nucleic acid aptamer-specific recognition with G-quadruplex/hemin DNAzyme (G4-DNAzyme), thereby addressing potential public health risks posed by heavy metal contamination. A dual-strand probe containing T-Hg2+-T coordination motifs was designed. Hg2?-triggered conformational transition liberated the G-quadruplex domain, which subsequently bound hemin to form catalytically active G4-DNAzyme. This enzyme-mimetic complex amplified signals by oxidizing o-phenylenediamine (OPD) into the fluorescent product 2,3-diaminophenazine (DAP). The biosensor exhibited a linear response to Hg2+ within 0.1~1.2 μg/L, achieving a limit of detection (LOD) of 0.1 μg/L. Validation using real-world samples (Xiangjiang River water, municipal tap water, and pond water) demonstrated satisfactory recoveries (93~107%) and precision (relative standard deviation: 0.58–8.33%). This method combines high sensitivity, cost-effectiveness, and practicability, requiring no complex pretreatment. It enables reliable monitoring of Hg2+ contamination in drinking water sources, providing critical technical support for the prevention of mercury exposure-related neurotoxicity, hepatorenal damage, and other public health hazards. |
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