| 孔祥君,陈玉妹,林英武,杜可杰.金属卟啉重组蛋白的光活性与光稳定性研究[J].南华大学学报(自然科学版),2023,(3):65~70, 98.[KONG Xiangjun,CHEN Yumei,LIN Yingwu,DU Kejie.Study on the Photoactivity and Photostability of Metalloporphyrin Recombinant Protein[J].Journal of University of South China(Science and Technology),2023,(3):65~70, 98.] |
| 金属卟啉重组蛋白的光活性与光稳定性研究 |
| Study on the Photoactivity and Photostability of Metalloporphyrin Recombinant Protein |
| 投稿时间：2022-12-08 |
| DOI： |
| 中文关键词: 肌红蛋白重组 亚甲基蓝 光催化 光稳定性 |
| 英文关键词:Reconstituted myoglobin methylene blue photocatalysis photostability |
| 基金项目:国家自然科学基金项目(21701081)；湖南省自然科学基金项目(2022JJ30485) |
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| 中文摘要: |
| 本研究以突变体肌红蛋白(myoglobin,Mb)F43H/H64A Mb为骨架,通过原卟啉锌(zinc-protoporphyrin,ZnPP)、原卟啉钴(cobalt-protoporphyrin,CoPP)替代蛋白天然辅基血红素(heme)的方式制备了两个新的重组肌红蛋白(reconstituted myoglobin,rMb),命名为ZnPP-F43H/H64A Mb和CoPP-F43H/H64AMb,并对其光稳定性和光催化降解染料活性进行了研究。结果显示,ZnPP-F43H/H64A Mb具备更好的光催化降解亚甲基蓝的能力,ZnPP-F43H/H64A具有光不稳性。 |
| 英文摘要: |
| In this work, two reconstituted myoglobins (rMb), ZnPP-F43H/H64A Mb and CoPP-F43H/H64A Mb, were prepared by replacing the natural heme of F43H/H64A Mb with zinc protoporphyrin (ZnPP) and cobalt protoporphyrin (CoPP). Their photostability and photocatalytic activity for dye degradation were also studied. The results showed that although ZnPP-F43H/H64A Mb has a certain ability of photocatalytic degradation of methylene blue, it was also photounstable. |
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