| 向亨芳,刘姣,杜可杰,林英武.人工脱色过氧化物酶催化降解苋菜红活性研究[J].南华大学学报(自然科学版),2021,(5):92~96.[XIANG Hengfang,LIU Jiao,DU Kejie,LIN Yingwu.Degradation of Amaranth Catalyzed by Artificial Decolorizing Peroxidase[J].Journal of University of South China(Science and Technology),2021,(5):92~96.] |
| 人工脱色过氧化物酶催化降解苋菜红活性研究 |
| Degradation of Amaranth Catalyzed by Artificial Decolorizing Peroxidase |
| 投稿时间：2021-03-23 |
| DOI： |
| 中文关键词: 苋菜红 人工脱色过氧化物酶 突变肌红蛋白 |
| 英文关键词:amaranth artificial dye-decolorizing peroxidases myoglobin mutants |
| 基金项目:国家自然科学基金项目(21701081;21977042) |
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| 中文摘要: |
| 以肌红蛋白(Mb)为骨架构建并纯化了三突变体蛋白F43Y/F138W/P88W Mb,双突变体蛋白F43Y/F138W Mb及单突变体蛋白 F43Y Mb,并对三个突变体蛋白的苋菜红(amaranth)脱色降解活性进行了研究。催化活性及酶动力学研究结果显示,相比于F43Y/F138W Mb和F43Y Mb,三突变体F43Y/F138W/P88W Mb的苋菜红催化效率最高,这表明色氨酸对人工脱色过氧化物酶的活性起了重要作用。该研究不仅为今后的血红素蛋白设计提供了理论依据,同时也表明人工酶在染料废水处理方面具有潜在的应用价值。 |
| 英文摘要: |
| Dye-decolorizing peroxidases (DyPs) are a new family of heme peroxidases and have received much attention recently. This study designed and puried a third generation artificial dye-decolorizing peroxidases (DyPs) in myoglobin by introduction of Tyr43, Trp138 in the heme pocket and Trp88 on the protein surface, named F43Y/F138W/P88W Mb. The third generation of artificial DyP was shown to exhibit a catalytic efficiency for amaranth exceeding that of the mutants, F43Y/F138W Mb and F43Y Mb. This study not only provides clues for heme protein design but also suggests that the artificial DyP has potential applications for bioremediation in the future. |
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