| 杨航,胡南,张辉,丁德馨.一种检测电芬顿体系下DNA损伤的电化学生物传感器的研制[J].南华大学学报(自然科学版),2020,34(6):24~29.[YANG Hang,HU Nan,ZHANG Hui,DING Dexin.Construction of an Electrochemical Biosensor for Detecting DNA Damage in the ElectroFenton System[J].Journal of University of South China(Science and Technology),2020,34(6):24~29.] |
| 一种检测电芬顿体系下DNA损伤的电化学生物传感器的研制 |
| Construction of an Electrochemical Biosensor for Detecting DNA Damage in the ElectroFenton System |
| 投稿时间：2020-06-29 |
| DOI： |
| 中文关键词: 电芬顿体系 DNA损伤 rGO/Fe3O4复合材料 电化学生物传感器 |
| 英文关键词:ElectroFenton system DNAdamage rGO/Fe3O4 composite material electrochemical biosensor |
| 基金项目:国防基础科研项目(JCKY2016403C001) |
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| 中文摘要: |
| 为了检测电芬顿体系下DNA的损伤,先采用石墨烯制备了一种致密的rGO/Fe3O4复合材料;再将复合材料和DNA修饰到玻碳电极上,利用电化学还原作用释放游离态Fe2+,并加入H2O2形成电芬顿体系;最后构建了一种检测电芬顿体系下DNA损伤的新型电化学生物传感器。检测结果表明,检测DNA损伤的最佳时间为30 min,最佳pH值为7.0。 |
| 英文摘要: |
| In order to detect the DNA damage in the electrofentonsystem,a compact rGO/Fe3O4 composite material was prepared with graphene at first.Then the composite material and DNA were modified onto glassy carbon electrode,and the free state Fe2+ was released by electrochemical reduction,and H2O2 was added to form an ElectroFenton system.Finally,a novel electrochemical biosensor was constructed to detect DNA damage in the ElectroFentonsystem.The experimental results showed that the optimal detecting time of DNA damage was 30 min,and the optimal pH value was 7.0. |
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