| 李娜梅,杨飒,宁倩,黄灿,黄文,贺子宁,贺冬秀,喻翠云.半乳糖化壳聚糖—氟尿嘧啶偶合物纳米给药体系的制备、表征及体外性能研究[J].南华大学学报(自然科学版),2015,29(3):82~86, 91.[LI Na-mei,YANG Sa,NING Qian,HUANG Can,HUANG Wen,HE Zi-ning,HE Dong-xiu,YU Cui-yun.Fabrication,Characterization and in Vitro Performance Evaluation of GC-FUA Based Nanoparticles Delivery System[J].Journal of University of South China(Science and Technology),2015,29(3):82~86, 91.] |
| 半乳糖化壳聚糖—氟尿嘧啶偶合物纳米给药体系的制备、表征及体外性能研究 |
| Fabrication,Characterization and in Vitro Performance Evaluation of GC-FUA Based Nanoparticles Delivery System |
| 投稿时间：2015-03-10 |
| DOI： |
| 中文关键词: GC-FUA纳米给药体系 5-Fu 缓释 HepG2 |
| 英文关键词:GC-FUA nano-delivery systems 5-Fu sustained release HepG2 |
| 基金项目:国家自然科学基金资助项目(81471177)；湖南省科学技术厅基金资助项目(2014GK3082);南华大学抗肿瘤药物创新团队；南华大学青年英才计划 |
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| 中文摘要: |
| 以预先合成的半乳糖化壳聚糖—氟尿嘧啶偶合物(GC-FUA)为原料,采用离子交联法制备出了GC-FUA纳米给药体系,并结合单因素分析法筛选出其最佳制备条件为GC-FUA浓度2.0 mg/mL,TPP浓度1.0 mg/mL,pH值4.5,GC-FUA/TPP质量比12∶1.应用SEM、UV等表征该纳米给药体系多呈球形,大小较均一,且具有缓释性能；采用 MTT 法观察 5-Fu、物理包封5-Fu GC纳米粒子、GC-FUA纳米粒子对HepG2细胞的增殖抑制作用.结果表明三者对 HepG2细胞的增殖具有明显抑制作用,且呈剂量依赖性,GC-FUA纳米粒子作用较 5-Fu和物理包封5-Fu GC纳米粒子明显增强. |
| 英文摘要: |
| In this experiment,N-galactosylated-chitosan-5-fluorouracil acetic acid conjugate (GC- FUA) which we previously synthesized nanoparticles,were produced by ionic crosslinking method based on modified ionic gelation of tripolyphosphate (TPP) with GC-FUA.The effects of GC-FUA concerntration,TPP concerntration,pH value,etc.on the preparation of GC-FUA nanoparticles were evaluated.And results were when GC-FUA concerntration was 2.0 mg/mL,TPP concerntration was 1.0 mg/mL,pH value was 4.5,GC-FUA/TPP mass ratio was 12∶1,opalescent solution of stable GC-FUA nanoparticles could be easily obtained.GC-FUA nanoparticles exhibited regularly spherical shapes,with a smooth surface and uniform size,and has a slow release properties which were confirmed by SEM and Nano ZS.Cytotoxicity study (MTT) in HepG2 cell lines demonstrated that the resulting GC-FUA nanoparticles were more potent in killing cancer cells,compared to free 5-fluorouracil (5-Fu) and 5-Fu-loaded GC nanoparticles. |
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