| 曾涛涛,李冬,曾辉平,仲航,张杰.两株好氧氨氧化菌(AOB)的分离纯化及菌属鉴定[J].南华大学学报(自然科学版),2014,28(4):23~27.[ZENG Tao-tao,LI Dong,ZENG Hui-ping,ZHONG Hang,ZHANG Jie.Isolation,Purification and Species Identification of AerobicAmmonia-Oxidizing Bacteria (AOB)[J].Journal of University of South China(Science and Technology),2014,28(4):23~27.] |
| 两株好氧氨氧化菌(AOB)的分离纯化及菌属鉴定 |
| Isolation,Purification and Species Identification of AerobicAmmonia-Oxidizing Bacteria (AOB) |
| 投稿时间：2014-05-22 |
| DOI： |
| 中文关键词: 好氧氨氧化菌 分离 鉴定 生物脱氮 |
| 英文关键词:Aerobic ammonia-oxidizing bacteria isolation identification biological nitrogen removal |
| 基金项目:国家自然科学基金资助项目(51408293);中国博士后基金资助项目(2014M562114);南华大学博士科研启动基金资助项目(2013XQD10) |
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| 中文摘要: |
| 在生物脱氮过程中,好氧氨氧化细菌(AOB)发挥重要作用.本研究通过梯度稀释培养、平板划线分离进行AOB分离纯化,获得两株氨氧化能力较强的AOB菌株A2和A7,它们能将培养液中的大部分氨氮氧化.对A2和A7菌株进行革兰氏染色,并通过光学显微镜及扫描电镜观察细胞形态,发现A2和A7均为革兰氏阴性菌,细胞呈杆状,长为1~1.5 μm,宽约0.5 μm.生理生化实验表明,A2和A7属于自养微生物,不能利用有机碳源.系统发育分析表明,A2和A7均为亚硝化单胞菌(Nitrosomonas). |
| 英文摘要: |
| Aerobic ammonia-oxidizing bacteria (AOB) play important roles in biological nitrogen removal process.In this study,gradient dilution of domestication liquid and streaking plate methods were used for AOB isolation and purification.Two strains of AOB,named A2 and A7,were obtained from culture medium,which contributed most ammonia oxidation.After gram stain,detected by optical microscopy and scanning electron microscopy,A2 and A7 were both found as gram-negative and rod-shaped bacteria,with length of 1~1.5 μm and width of 0.5 μm.A2 and A7 were autotrophic microorganisms since organic carbon couldn’t be ultized.Phylogenetic analysis showed that the species of A2 and A7 were Nitrosomonas. |
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