| 王慧,沈鹏飞,曾荣今*.枸骨根中三萜类物质含量的测定[J].南华大学学报(自然科学版),2011,25(4):105~108.[WANG Hui1,2,SHEN Peng-fei2,ZENG Rong-jin1,2*.Determination of Triterpenes from the Root of Ilex Cornuta[J].Journal of University of South China(Science and Technology),2011,25(4):105~108.] |
| 枸骨根中三萜类物质含量的测定 |
| Determination of Triterpenes from the Root of Ilex Cornuta |
| 投稿时间：2011-10-19 |
| DOI： |
| 中文关键词: 枸骨根 总三萜 分光光度法 熊果酸 |
| 英文关键词:the root of Ilex cornuta total triterpenes spectrophotometry ursolic acid |
| 基金项目: |
|
| 摘要点击次数: 777 |
| 全文下载次数: 1183 |
| 中文摘要: |
| 本文以采集于湘潭县的枸骨根为材料，选用熊果酸为对照品，5%香草醛—冰乙酸和高氯酸为显色剂，以分光光度法测定枸骨根中总三萜的含量.实验结果表明：在549 nm波长下，标准品在4.4～26.4 mg/L范围内，吸光度与含量呈良好的线性关系，其回归方程为：y=0.0357x-0.0809,R2=0.9984.样品吸光度在5～40 min内相对比较稳定，其三萜含量为3.20%，RSD值为0.31%.该方法操作简便，快速，灵敏度高，重复性好. |
| 英文摘要: |
| The total triterpenoids in the root of Ilex cornuta was quantified by spectrometry method using ursolic acid as standard and 5% vanillin-glacial acetic acid and perchloric acid as color-developing agents.The results showed that the content of the standard was well correlated to its optical density at 549 nm,the calibration curve was linear( R2=0.9984)in the range of 4.4～ 26.4 mg/L within 5～40 min and the curve was y=0.0357x-0.0809.The above method for the quantification was proved to have the advantages of simple operation,rapidity,high sensitivity and fine repetition. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|