| 向艳，杨慧仙*，于军晖，杨胜园，朱博.丹酰氯荧光猝灭法测定芹菜中芹菜素含量[J].南华大学学报(自然科学版),2011,25(1):87~91.[XIANG Yan,YANG Hui-xian*,YU Jun-hui,YANG Sheng-yuan,ZHU Bo.Determination of Apigenin in Celery byDansyl chloride Fluorescence Quenching Spectrophotometry[J].Journal of University of South China(Science and Technology),2011,25(1):87~91.] |
| 丹酰氯荧光猝灭法测定芹菜中芹菜素含量 |
| Determination of Apigenin in Celery byDansyl chloride Fluorescence Quenching Spectrophotometry |
| 投稿时间：2010-09-25 |
| DOI： |
| 中文关键词: 芹菜素 丹酰氯 荧光猝灭 |
| 英文关键词:apigenin dansyl chloride fluorescence quenching |
| 基金项目:湖南省教育厅基金资助项目（07C634） |
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| 中文摘要: |
| 在pH 7～8的弱碱性条件下，丹酰氯可与芹菜素反应生成一种酚酯化合物，使丹酰氯的内源性荧光发生猝灭，其猝灭程度（ΔF=F0-F）与加入芹菜素的量在一定范围内呈良好的线性关系.在优化的条件下，体系ΔF与芹菜素的浓度在6.40×10-7 mol/L～5.92×10-5 mol/L范围内线性关系良好，线性回归方程为ΔF=112.18CAp（×10-5 mol/L）-1.25，相关系数r=0.998 9.检出限为1.92×10-7 mol/L，RSD为2.6%～4.1%，加标回收率为98.1%～101.5%.本方法简便、快速，用于芹菜中芹菜素的含量测定结果满意. |
| 英文摘要: |
| After the phenol ester compound generated by apigenin reacting with dansyl chloride in weakly alkaline medium,the endogenous fluorescence of dansyl chloride was quenched,and there was a good linear relationship between the quenching value(ΔF=F0-F) and the concentration of apigenin.And under the optimal conditions,the linear range of calibration curve for determination of apigenin was 6.40×10-7～5.92×10-5 mol/L,and the linear regression equation was ΔF= 112.18CAp（×10-5 mol/L）-1.25 with correlation coefficient 0.9989 and the detection limit for apigenin was 1.92×10-7 mol/L.The method was than 4.1% and the recoveries for apigenin from 98.1 %～101.5 %.The developed method was sensitive and had low detection limit and could be suitable for determining apigenin in celery. |
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