| 朱莉,蔡清平,张馨.配合物[Ni(C12N3H9)2(C3H4N2)·Cl]·(ClO4)与DNA相互作用的研究[J].南华大学学报(自然科学版),2009,23(3):46~52.[ZHU Li，CAI Qing-ping，ZHANG Xin.Study on the Interaction between Complex [Ni(C12N3H9)2(C3H4N2)·Cl]·(ClO4) and DNA[J].Journal of University of South China(Science and Technology),2009,23(3):46~52.] |
| 配合物[Ni(C12N3H9)2(C3H4N2)·Cl]·(ClO4)与DNA相互作用的研究 |
| Study on the Interaction between Complex [Ni(C12N3H9)2(C3H4N2)·Cl]·(ClO4) and DNA |
| 投稿时间：2009-05-18 |
| DOI： |
| 中文关键词: 镊(Ⅱ)配合物；合成 DNA；Scatchard图；插入结合；静电结合 |
| 英文关键词:Ni(Ⅱ)complex synthesis DNA Scatchard partial intercalation electricity attraction |
| 基金项目:湖南省自然科学基金资助项目(02JJY2018;06JJ30004;07JJ5005) |
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| 中文摘要: |
| 用溶液法合成了镍(Ⅱ)与2-(2-吡啶)苯并咪唑(HPBM)，咪唑的混配配合物，其分子式为[Ni(C12N3H9)2(C3H4N2)·Cl]·(ClO4).在接近生理条件pH=7.2下，研究它与DNA作用的电子吸收光谱、荧光光谱，求得配合物与DNA的结合常数，研究了配合物对EB-DNA体系的Scatchard图，结果表明配合物与DNA作用既存在部分插入结合模式又存在静电结合模式. |
| 英文摘要: |
| This paper introduces that the microwave synthesis of 2-(2-pyridyl)-benzimidazole and solvothermal reaction of Ni(Ⅱ)complex with 2-(2-pyridyl)-benzimidazole and imidazole,the structures of the complex may be [Ni(C12N3H9)2(C3H4N2)·Cl]·(ClO4).The interaction of the complex and DNA has been investigated by using UV spectra and fluorescent spectra.The emission intensity was quenched as the complex was added to the EB-DNA system.The apparent binding constants of the complex and DNA was obtained at physiological pH(pH＝7.2).The results suggest that the interaction of Ni(II)complex and DNA belong to both partial intercalation and static electricity attraction mode. |
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