| 肖锡林,廖力夫,肖静水,匡汀,刘传湘,吴抗抗,赵俊,李刚.三聚氰胺的HPLC-DAD检测新方法研究[J].南华大学学报(自然科学版),2009,23(2):83~86.[XIAO Xi-lin1,LIAO Li-fu1,XIAO Jing-shui1,KUANG Ting1,LIU Chuan-Xiang1,WU Kang-kang2,ZHAO Jun1,LI Gang1.Study of New Determination Method of Melamine by High Performance Liquid Chromatography with Diode Array Detectors[J].Journal of University of South China(Science and Technology),2009,23(2):83~86.] |
| 三聚氰胺的HPLC-DAD检测新方法研究 |
| Study of New Determination Method of Melamine by High Performance Liquid Chromatography with Diode Array Detectors |
| 投稿时间：2009-01-12 |
| DOI： |
| 中文关键词: 三聚氰胺 测定 HPLC-DAD |
| 英文关键词:melamine determination high performance liquid chromatography-diode array detector (HPLC-DAD) |
| 基金项目:国家自然科学基金资助项目（20877038）;湖南省卫生厅科研基金资助项目（B2006-103） |
| 作者 | 单位 | | 肖锡林1,廖力夫1,肖静水1,匡汀1,刘传湘1,吴抗抗2,赵俊1,李刚1 | 1．南华大学 化学化工学院,湖南 衡阳,421001
2.湖南大学 化学化工学院,湖南 长沙 410082 |
|
| 摘要点击次数: 811 |
| 全文下载次数: 1290 |
| 中文摘要: |
| 待测样品以适量1%三氯乙酸溶液提取,2%乙酸铅溶液沉淀蛋白,离心后上清液经0.45 μm滤膜过滤后直接进行HPLC分析.据此建立了蛋白类保健食品中非法添加三聚氰胺的高效液相色谱-二级管阵列检测新方法.方法的线性范围为0.02～2.0 mg/L,r =0.999 5,检出限为0.14 ng.该方法简便、快速,灵敏度高,可准确排除阴性干扰,易推广应用. |
| 英文摘要: |
| The sample was extracted by 1% TCA solution,and 2% lead acetate solution for protein precipitation.As centrifuged,supernatant layer was filtrated with 0.45 μm membrane for HPLC analysis.The linear range for the method was 0.02～2.0 mg/L,r=0.999 5,and detection limit for the method was 0.14 ng.The method is applicable for the determination of melamine in nitrogenous health food products.Spectrum of diode array detector is easy to exclude negative interference.Results show that the method is simple,rapid,sensitive and easy to be applied. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|