| 于军晖,杨慧仙,杨胜园,黄弦,姜乐.钕-肉桂酸-邻菲罗啉三元配合物与DNA体系荧光光谱研究及其应用[J].南华大学学报(自然科学版),2009,23(1):92~95.[YU Jun-hui,YANG Hui-xian,YANG Sheng-yuan,HUANG Xian,JIANG Le.Studies on Interaction Between Ternary Rare Earth Complexes of Cinnamic Acid and Phenathroline with DNA by Fluorescent Spectrum and Its Application[J].Journal of University of South China(Science and Technology),2009,23(1):92~95.] |
| 钕-肉桂酸-邻菲罗啉三元配合物与DNA体系荧光光谱研究及其应用 |
| Studies on Interaction Between Ternary Rare Earth Complexes of Cinnamic Acid and Phenathroline with DNA by Fluorescent Spectrum and Its Application |
| 投稿时间：2008-12-20 |
| DOI： |
| 中文关键词: DNA 钕 肉桂酸 邻菲罗啉 荧光敏化作用 |
| 英文关键词:DNA Nd Cinnamic Acid Phenanthroline fluorescence sensitization effect |
| 基金项目:湖南省教育厅基金资助项目（03C418） |
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| 中文摘要: |
| 研究了DNA对钕-肉桂酸-邻菲罗啉三元配合物的荧光敏化作用,并在此基础上建立了荧光法测定DNA的新方法.pH=8.0的Tris缓冲液中,DNA在0.23～5.68 μg/mL浓度范围内,稀土配合物-DNA体系的荧光强度值（F）与DNA浓度（CDNA）具有良好的线性关系,线性回归方程F=22.40CDNA＋83.88,相关系数r=0.997,检出限为0.07 μg/mL,加标回收率为92.6%～103.2%,RSD＝1.85％～3.42％.结果表明,由于DNA的加入,使体系荧光强度增强,且加入DNA浓度和体系荧光强度间存在线性关系,表明配合物和DNA间存在插入作用. |
| 英文摘要: |
| A new Fluorescent Spectrum method for the determination of DNA with Ternary Rare Earth Complexes has been established under the special condition.The Fluorescent intensity was proporitional to the concentration of DNA in the range of 0.23～5.68 μg/mL with the detection limit of 0.07μg/mL.The average recovery was 92.6% ～103.2%,the RSD was 1.85%～3.42%.The fluorescence intensity value of complex of DNA and [Nd (phen)(cin)3(H2O)] has be heightened.That shows the effect of insert can be proved between DNA and the rare earth complexes. |
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