| 黄丽,龚豪,张春莲.miR-519b-3p调控IL-6/STAT3通路对宫颈癌细胞凋亡、迁移、侵袭的影响.[J].中南医学科学杂志.,2024,(1):56-59, 90. |
| miR-519b-3p调控IL-6/STAT3通路对宫颈癌细胞凋亡、迁移、侵袭的影响 |
| Effects of miR-519b-3p on the apoptosis, migration and invasion of cervical cancer cells by regulating IL-6/STAT3 pathway |
| 投稿时间：2023-06-16 修订日期：2023-12-15 |
| DOI：10.15972/j.cnki.43-1509/r.2024.01.012 |
| 中文关键词: 宫颈癌 miR-519b-3p 细胞凋亡 细胞迁移 细胞侵袭 IL-6/STAT3通路 [ |
| 英文关键词:cervical cancer miR-519b-3p apoptosis migration invasion IL-6/STAT3 pathway |
| 基金项目:十堰市科技局计划项目(20200234) |
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| 中文摘要: |
| 目的探讨miR-519b-3p调控IL-6/信号转导和转录激活因子3(STAT3)通路对宫颈癌细胞(SiHa)凋亡、迁移、侵袭的影响。 方法qRT-PCR检测miR-519b-3p在宫颈癌组织中的表达。SiHa细胞分为miR-NC组、miR-519b-3p组、miR-NC+RhIL-6组、miR-519b-3p+RhIL-6组。流式细胞术评估SiHa细胞凋亡率,Transwell实验检测细胞迁移和侵袭情况。Western blotting检测B细胞淋巴瘤-2基因(Bcl-2)、E-钙黏蛋白(E-cadherin)、N-cadherin、Bcl相关x蛋白(Bax)、STAT3和p-STAT3蛋白水平。 结果与癌旁组织比较,宫颈癌组织miR-519b-3p表达下调(P＜0.05)。SiHa细胞凋亡率、Bax和E-cadherin蛋白水平miR-519b-3p组高于miR-NC组,miR-NC+RhIL-6组低于miR-NC组,miR-519b-3p+RhIL-6组低于miR-519b-3p组(P＜0.05)。SiHa细胞迁移数、侵袭数、Bcl-2、N-cadherin、p-STAT3蛋白水平miR-519b-3p组低于miR-NC组,miR-NC+RhIL-6组高于miR-NC组,miR-519b-3p+RhIL-6组高于miR-519b-3p组(P＜0.05)。 结论miR-519b-3p通过抑制IL-6/STAT3通路来抑制宫颈癌细胞迁移和侵袭,并增加细胞凋亡。 |
| 英文摘要: |
| AimTo investigate the effect of miR-519b-3p on apoptosis, migration, and invasion of cervical cancer cells (SiHa) through the regulation of IL-6/signal transduction and transcription activating factor 3 (STAT3) pathway. MethodsqRT-PCR was used to detect the expression of miR-519b-3p in cervical cancer tissue. SiHa cells were divided into miR-NC group, miR-519b-3p group, miR-NC+RhIL-6 group, and miR-519b-3p+RhIL-6 group. Flow cytometry was used to evaluate the apoptosis rate of SiHa cells, while Transwell assay was used to detect cell migration and invasion. Western blotting was used to detect the levels of B-cell lymphomatoma-2 gene (Bcl-2), E-cadherin, N-cadherin, Bcl related x protein (Bax), STAT3, and p-STAT3 protein. ResultsCompared with adjacent tissues, the expression of miR-519b-3p in cervical cancer tissue was downregulated (P＜0.05). The apoptosis rate of SiHa cells, Bax and E-cadherin protein levels were higher in the miR-519b-3p group than in the miR-NC group, lower in the miR-NC+RhIL-6 group than in the miR-NC group, and lower in the miR-519b-3p+RhIL-6 group than in the miR-519b-3p group (P＜0.05). The migration number, invasion number, Bcl-2, N-cadherin, p-STAT3 protein levels of SiHa cells were lower in the miR-519b-3p group than in the miR-NC group, higher in the miR-NC+RhIL-6 group than in the miR-NC group, and higher in the miR-519b-3p+RhIL-6 group than in the miR-519b-3p group (P＜0.05). ConclusionmiR-519b-3p inhibits the migration and invasion of cervical cancer cells and increases cell apoptosis by inhibiting the IL-6/STAT3 pathway. |
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