| 章硕,夏红,刘芳,周志刚,姜浩,苏琦.沉默LIMK1抑制人胃癌MGC803细胞迁移与侵袭.[J].中南医学科学杂志.,2020,(1):65-71. |
| 沉默LIMK1抑制人胃癌MGC803细胞迁移与侵袭 |
| Silencing LIMK1 inhibits migration and invasion of human gastric cancer MGC803 cells |
| 投稿时间：2019-06-01 修订日期：2019-10-10 |
| DOI：10.15972/j.cnki.43-1509/r.2020.01.018 |
| 中文关键词: 人胃癌MGC803细胞 LIMK1 RNA干扰 增殖 迁移 侵袭 |
| 英文关键词:human gastric cancer MGC803 cells LIMK1 RNA interference migration invasion |
| 基金项目: |
| 作者 | 单位 | | 章硕 | 湖南省肿瘤细胞与分子病理学重点实验室,湖南省胃癌研究中心,南华大学肿瘤研究所, 湖南 衡阳 421001
株洲市中心医院肿瘤科,湖南 株洲 412007 | | 夏红 | 湖南省肿瘤细胞与分子病理学重点实验室,湖南省胃癌研究中心,南华大学肿瘤研究所, 湖南 衡阳 421001 | | 刘芳 | 湖南省肿瘤细胞与分子病理学重点实验室,湖南省胃癌研究中心,南华大学肿瘤研究所, 湖南 衡阳 421001 | | 周志刚 | 湖南省肿瘤细胞与分子病理学重点实验室,湖南省胃癌研究中心,南华大学肿瘤研究所, 湖南 衡阳 421001 | | 姜浩 | 湖南省肿瘤细胞与分子病理学重点实验室,湖南省胃癌研究中心,南华大学肿瘤研究所, 湖南 衡阳 421001 | | 苏琦 | 湖南省肿瘤细胞与分子病理学重点实验室,湖南省胃癌研究中心,南华大学肿瘤研究所, 湖南 衡阳 421001 |
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| 中文摘要: |
| 探讨沉默LIM激酶1(LIMK1)对人胃癌MGC803细胞迁移与侵袭的影响。采用RNA干扰技术沉默MGC803细胞LIMK1基因,RT-PCR和Western blot检测干扰效率,MTT、流式细胞术、细胞划痕和侵袭实验分别检测沉默LIMK1对MGC803细胞增殖、细胞周期、迁移与侵袭能力的影响。结果显示,成功构建稳定沉默LIMK1基因MGC803细胞。MTT显示,LIMK1沉默组细胞在24、48、72、96 h分别较对照组与空载体组的抑制率为61.0%、36.9%、12.1%、1.6%和54.7%、46.2%、13.5%、1.5%(P<0.05)。流式细胞术显示,沉默组G2/M期17.96%明显高于MGC803组11.45%和空载体组11.68%(P<0.05)。划痕实验显示,24 h后,沉默组划痕距离(155.9±7.6)较对照组(65.9±11.0)和空载体组(73.2±5.1)明显增加(P<0.05)。侵袭实验显示,沉默组穿膜细胞(25.1±1.3)较对照组(42.4±2.8)和空载体组(45.2±3.0)明显减少(P<0.05)。沉默LIMK1可抑制MGC803细胞增殖、迁移与侵袭和阻滞G2/M期。 |
| 英文摘要: |
| To investigate the effect of silent LIMK1 on migration and invasion of human gastric cancer MGC803 cells. RNA interference technology was used to silence the gene LIMK1 in MGC803 cells. Interference efficiency was detected by RT-PCR and Western blot. The effects of LIMK1 silencing on the proliferation, cell cycle, migration and invasion of MGC803 cells were detected by MTT, flow cytometry, cell scratching and invasion. The results showed that LIMK1 gene MGC803 cells were successfully constructed. MTT showed that the inhibition rates of limk1-silencing group at 24h, 48h, 72h and 96h were 61.0%, 36.9%, 12.1% and 1.6%, 54.7%, 46.2%, 13.5% and 1.5%, respectively, compared with the control group and the empty carrier group (P<0.05). Flow cytometry showed that 17.96% of G2/M phase in the silent group was significantly higher than 11.45% of MGC803 group and 11.68% of the empty Vector group (P<0.05). The scratch test showed that after 24h, the scratch distance of the silent group was(155.9±7.6), which was significantly increased compared with the control group (65.9±11.0) and the empty carrier group (73.2± 5.1) (P<0.05). The invasion test showed that transmembrane cells in the silent group were significantly reduced by(25.1±1.3)compared with those in the control group (42.4±2.8) and the empty Vector group (45.2±3.0) (P<0.05). It is indicated that silencing LIMK1 can inhibit the proliferation, migration and invasion of MGC803 cells and blocked G2/M phase. |
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