| 龙海旭,詹红艳,狄雯雯,唐美美.POMC基因敲除对高脂饮食诱导的肥胖雄性幼鼠睾丸功能的影响机制.[J].中南医学科学杂志.,2026,(1):25-29. |
| POMC基因敲除对高脂饮食诱导的肥胖雄性幼鼠睾丸功能的影响机制 |
| The mechanism of POMC gene knockout to affect testicular function in obese male young mice induced by high-fat diet obesity |
| 投稿时间：2025-01-05 修订日期：2025-11-11 |
| DOI：10.15972/j.cnki.43-1509/r.2026.01.005 |
| 中文关键词: 肥胖 POMC基因敲除 睾丸功能 高脂饮食 幼鼠 |
| 英文关键词:obesity POMC gene knockout testicular function high-fat diet young mice |
| 基金项目:湖南省卫生健康委卫生科研课题项目(W20243267) 作者简介:龙海旭,硕士,主治医师,研究方向为儿科疾病的中医治疗,E-mail为593218204@qq.com。 |
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| 中文摘要: |
| 目的探究阿黑皮素原(POMC)基因敲除对高脂饮食诱导的肥胖雄性幼鼠睾丸功能的影响机制。 方法将20只野生型C57BL/6雄性幼鼠随机均分为对照组(普通饲料喂养)与高脂饮食组(高脂饲料喂养),另10只POMC－/－幼鼠设为敲除POMC高脂组(高脂饲料喂养)。比较各组同时间幼鼠体质量,测量睾丸容积。采用HE、油红O染色检测各组睾丸组织病理结构与脂肪含量；Western blotting、qRT-PCR检测睾丸组织POMC、可卡因-苯丙胺调节转录肽(CART)蛋白及其mRNA水平；试剂盒检测血清睾酮与血脂[甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDLC)、高密度脂蛋白胆固醇(HDLC)]水平；TUNEL染色检测各组睾丸组织细胞凋亡水平。 结果与对照组比较,高脂饮食组、敲除POMC高脂组在第8、第12周时的体质量、睾丸组织红染的中性脂肪增加(P＜0.05),睾丸组织生精小管生殖细胞层减少(P＜0.05),睾丸容积、睾酮、HDLC和POMC、CART蛋白及其mRNA表达水平降低(P＜0.05),TC、TG、LDLC水平及细胞凋亡水平升高(P＜0.05)。与高脂饮食组比较,敲除POMC高脂组幼鼠在第8、第12周时的体质量、睾丸组织红染的中性脂肪增加(P＜0.05),睾丸组织生精小管生殖细胞层减少(P＜0.05),睾丸容积、睾酮、HDLC和POMC、CART蛋白及其mRNA水平降低(P＜0.05),TC、TG、LDLC及细胞凋亡水平升高(P＜0.05)。 结论POMC基因敲除显著加重高脂饮食诱导的肥胖雄性幼鼠睾丸功能损伤,其机制可能与POMC/CART信号通路受到抑制,进而导致代谢失调与细胞凋亡增加有关。 |
| 英文摘要: |
| AimTo explore the mechanism of pro-opiomelanocortin (POMC) gene knockout to affect testicular function in obse young mice induced by high-fat diet obesity. MethodsTwenty wild-type C57BL/6 male offspring mice were randomly and equally divided into a control group (fed a standard diet) and a high-fat diet group (fed a high-fat diet), while another ten POMC－/－ pups were used as the knockout POMC high-fat group (fed a high-fat diet). The body weight of neonatal mice among different groups at the same time were compared, the testicular volume was measured. HE staining and oil red O staining were used to detect the histopathological structure and fat content of testicular tissues in each group; Western blotting and qRT-PCR were used to detect the protein and mRNA expression levels of POMC and cocaine- and amphetamine-regulated transcript (CART) in testicular tissues. The reagent kit is used to detect the levels of serum testosterone and blood lipids [ triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDLC), and high-density lipoprotein cholesterol (HDLC)]; TUNEL staining was used to detect the apoptosis level of testicular tissue cells in each group. ResultsCompared with the control group, the high-fat diet group and the knockout POMC high-fat group showed an increase in body weight at weeks 8 and 12, and red-stained neutral fat in testicular tissue (P＜0.05), while a decrease in germ cell layer of seminiferous tubules in testicular tissue (P＜0.05), a decrease in testicular volume, testosterone, HDLC, and POMC, CART protein and their mRNA expression levels (P＜0.05), and an increase in TC, TG, LDLC levels and cell apoptosis levels (P＜0.05). Compared with the high-fat diet group, the weight at weeks 8 and 12, and red-stained neutral fat in testicular tissue of the knockout POMC high-fat group were increased (P＜0.05), while the germ cell layer of seminiferous tubules in testicular tissue were decreased (P＜0.05), and testicular volume, testosterone, HDLC, and POMC, CART protein and their mRNA levels were also decreased (P＜0.05), in addition, TC, TG, LDLC and cell apoptosis levels were increased (P＜0.05). ConclusionKnockout of POMC gene significantly exacerbates testicular dysfunction induced by high-fat diet in obese male young mice, and its mechanism may be related to the inhibition of POMC/CART signaling pathway, leading to metabolic disorders and increased cell apoptosis. |
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