| 张佳佳,赵剑,刘冬梅,韩冰.miR-21与CCL20在结直肠癌微环境中的表达及其与临床病理特征的关系.[J].中南医学科学杂志.,2025,(6):1019-1022. |
| miR-21与CCL20在结直肠癌微环境中的表达及其与临床病理特征的关系 |
| Expression of miR-21 and CCL20 in the colorectal cancer microenvironment and their correlation with clinicopathological characteristics |
| 投稿时间：2025-02-10 修订日期：2025-09-22 |
| DOI：10.15972/j.cnki.43-1509/r.2025.06.019 |
| 中文关键词: miR-21 CCL20 结直肠癌 肿瘤微环境 |
| 英文关键词:miR-21 CCL20 colorectal cancer tumor microenvironment |
| 基金项目:白求恩公益基金会资助项目(HSKY-017) |
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| 中文摘要: |
| 目的探究miR-21与CCL20在结直肠癌微环境中的表达及其与临床病理特征的关系。 方法选择结直肠癌(CRC)患者96例,取肿瘤组织和癌旁正常组织,检测样本中CCL20 mRNA和蛋白表达情况、miR-21表达情况,并比较不同病理特征患者CCL20和miR-21的表达差异。采用免疫组化和原位杂交对样本中的CCL20和miR-21进行定位,比较其差异。 结果与癌旁正常组织相比,CRC肿瘤组织中CCL20(mRNA和蛋白)和miR-21表达水平均显著上调(P＜0.05)。CCL20 mRNA、蛋白及miR-21的表达水平均在中晚期患者的肿瘤组织中显著高于早期患者(均P＜0.05)。细胞定位结果显示,CCL20主要在肿瘤微环境中的浸润免疫细胞(如巨噬细胞/淋巴细胞)中呈强阳性,而在肿瘤细胞中表达较低；miR-21主要在肿瘤相关成纤维细胞中呈强阳性,在肿瘤细胞中亦表达较低。 结论miR-21和CCL20在CRC组织中均显著上调,与CRC分期和分化相关；CCL-20主要表达于浸润免疫细胞,miR-21主要表达于肿瘤相关成纤维细胞。 |
| 英文摘要: |
| AimTo investigate the expression of miR-21 and CCL20 in the colorectal cancer microenvironment and their correlation with clinicopathological characteristics. MethodsNinety-six patients with colorectal cancer (CRC) were selected. Tumor tissues and adjacent normal tissues were collected. The expression of CCL20 mRNA and protein, as well as miR-21, was detected and compared among patients with different pathological features. Immunohistochemistry and in situ hybridization were used to localize CCL20 and miR-21, and their differences were compared. ResultsCompared with adjacent normal tissues, the expression levels of CCL20 (mRNA and protein) and miR-21 were significantly upregulated in CRC tumor tissues (all P＜0.05). The expression levels of CCL20 mRNA, protein, and miR-21 were significantly higher in tumor tissues of patients with advanced stages than in those with early stages (all P＜0.05). Cellular localization showed that CCL20 was strongly positive in infiltrating immune cells (such as macrophages and lymphocytes) within the tumor microenvironment but weakly expressed in tumor cells; miR-21 was strongly positive in tumor-associated fibroblasts but weakly expressed in tumor cells. ConclusionmiR-21 and CCL20 are significantly upregulated in CRC tissues and are associated with CRC stage and differentiation. CCL20 is mainly expressed in infiltrating immune cells, whereas miR-21 is mainly expressed in tumor-associated fibroblasts. |
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