| 陈守涛,沈天天,樊婷伟,查曹兵,刘改玲,张燕.间歇性禁食对血管性痴呆大鼠记忆功能的影响机制.[J].中南医学科学杂志.,2025,(6):973-976. |
| 间歇性禁食对血管性痴呆大鼠记忆功能的影响机制 |
| Influential mechanism of intermittent fasting on memory function in vascular dementia rats |
| 投稿时间：2024-12-20 修订日期：2025-07-12 |
| DOI：10.15972/j.cnki.43-1509/r.2025.06.007 |
| 中文关键词: 血管性痴呆 间歇性禁食 水迷宫 尼氏染色 免疫组化 大鼠 记忆功能 |
| 英文关键词:VD IF water maze Nissl staining immunohistochemistry rats memory function |
| 基金项目:“十四五”国家重点研发计划项目(2021YFC2500103);国网上海市电力公司科技项目(SGTYHT/23-JS-001) |
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| 中文摘要: |
| 目的探讨间歇性禁食(IF)对血管性痴呆(VD)大鼠记忆功能的影响机制。 方法将24只雄性SD大鼠随机均分为随意喂养对照组(Con-AL组)、随意喂养VD组(VD-AL组)和IF喂养VD组(VD-IF组)。Con-AL组行假手术并自由摄食,VD-AL组、VD-IF组通过手术建立VD模型后分别自由摄食和接受IF干预。术后5周,比较各组大鼠体质量；采用Morris水迷宫实验评估各组大鼠记忆功能；采用尼氏染色评估各组大鼠海马神经元缺失情况；采用LFB染色评估各组大鼠胼胝体白质损伤情况；采用免疫组化评估各组大鼠海马星形胶质细胞活化情况。 结果术后5周,各组大鼠体质量为VD-IF组＜VD-AL组＜Con-AL组(P＜0.05)。与Con-AL组比较,VD-AL组大鼠目标象限停留时间和平台穿越次数减少(P＜0.05),神经元缺失增加(P＜0.05)；VD-IF组逆转VD-AL组上述指标水平(P＜0.05)。与Con-AL组比较,VD-AL组、VD-IF组大鼠白质损伤加重(P＜0.05)。海马阳性星形胶质细胞数量为Con-AL组＜VD-AL组＜VD-IF组(P＜0.05)。Pearson相关分析显示,VD-IF组大鼠海马CA1区神经元数量与目标象限停留时间、平台穿越次数呈正相关(P＜0.05)。 结论IF干预可以改善VD大鼠记忆功能,可能与抑制大鼠海马神经元缺失,并增加星形胶质细胞活化有关。 |
| 英文摘要: |
| AimTo explore the influential mechanism of intermittent fasting (IF) on memory function in vascular dementia (VD) rats. MethodsTwenty four male SD rats were randomly divided into the the random feeding control group (Con-AL group), the random feeding of VD group (VD-AL group), and the IF feeding VD group(VD-IF group). The Con-AL group underwent sham surgery and had free feeding, whereas the VD-AL group, and VD-IF group as VD models through surgery received free feeding and IF intervention, respectively. Five weeks after surgery, the body weight of rats in each group were compared, the memory function of rats in each group was evaluated by Morris water maze, the hippocampal neuron loss was evaluated by Nissl staining, the white matter injury of corpus callosum was evaluated by LFB staining, and the hippocampal glial cell activation was evaluated by immunohistochemistry. ResultsFive weeks after surgery, the body mass of rats in each group was in an order of VD-IF group＜VD-AL group＜Con-AL group (P＜0.05). Compared with the Con-AL group,the target quadrant dwell time and platform crossing frequency of VD-AL group rats were reduced (P＜0.05), while neuronal loss were increased (P＜0.05). The VD-IF group reversed the levels of the above indicators in the VD-AL group (P＜0.05). Compared with the Con-AL group, the VD-AL group and VD-IF group showed aggravated white matter damage in rats(P＜0.05). The number of positive astrocytes in the hippocampus was in an order of the Con-AL group＜VD-AL group＜VD-IF group (P＜0.05). Pearson correlation analysis showed that the number of neurons in the hippocampal CA1 region of VD-IF group rats was positively correlated with target quadrant dwell time and platform crossing frequency (P＜0.05). ConclusionIF can improve the memory function of VD rats, which may be related to the inhibition of loss of hippocampal neurons and increase of activation of hippocampal astrocytes in rats. |
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