| 王素梅,袁云菲,张蔚玲,郭改莉,梁燕,徐超,李森.黄芩多糖对COX-2/PGE2/Akt通路及溃疡性结肠炎的影响.[J].中南医学科学杂志.,2025,(6):969-972. |
| 黄芩多糖对COX-2/PGE2/Akt通路及溃疡性结肠炎的影响 |
| Effects of scutellaria baicalensis polysaccharides on the COX-2/PGE2/Akt pathway and ulcerative colitis |
| 投稿时间：2024-12-24 修订日期：2025-09-12 |
| DOI：10.15972/j.cnki.43-1509/r.2025.06.006 |
| 中文关键词: 溃疡性结肠炎 黄芩多糖 COX-2/PGE2/Akt通路 |
| 英文关键词:ulcerative colitis scutellaria baicalensis polysaccharides COX-2/PGE2/Akt pathway |
| 基金项目:河北省医学科学研究课题计划项目(20241263) |
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| 中文摘要: |
| 目的基于环氧合酶-2(COX-2)/前列腺素E2(PGE2)/蛋白激酶B(Akt)通路探究黄芩多糖对溃疡性结肠炎(UC)大鼠的影响。 方法将30只大鼠随机均分为对照组、UC组、黄芩多糖组。HE染色检测各组大鼠结肠组织的病理变化。Western blotting、qRT-PCR分别检测各组结肠组织COX-2、PGE2、Akt、闭合蛋白(Occludin)、紧密连接蛋白-1(ZO-1)蛋白和mRNA水平。ELISA试剂盒检测各组血清炎症因子水平。 结果UC组大鼠结肠黏膜结构破坏,腺体丢失,炎症细胞浸润,黄芩多糖组大鼠结肠黏膜结构完整,炎症细胞较UC组浸润减少。与对照组比较,UC组大鼠结肠组织COX-2、PGE2、Akt蛋白及mRNA表达水平以及血清炎症因子水平升高(P＜0.05),Occludin,ZO-1蛋白及mRNA表达水平降低(P＜0.05)。与UC组比较,黄芩多糖组大鼠结肠组织COX-2、PGE2、Akt蛋白及mRNA表达水平以及血清炎症因子水平降低(P＜0.05),Occludin、ZO-1蛋白及mRNA表达水平升高(P＜0.05)。 结论黄芩多糖可能通过抑制COX-2/PGE2/Akt信号通路,改善了UC的肠道黏膜屏障功能,减轻了炎性损伤。 |
| 英文摘要: |
| AimTo explore the effects of scutellaria baicalensis polysaccharides on ulcerative colitis (UC) rats based on the mechanism of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2)/protein kinase B (Akt) pathway. MethodsThirty rats were randomly divided into the control group, the UC group and the scutellaria baicalensis polysaccharides group. HE staining was used to detect the pathological changes in the colon tissues of rats in each group. Western blotting and qRT-PCR were used to detect the protein and mRNA levels of COX-2, PGE2, Akt, Occludin, and zonula occludens-1 (ZO-1) in colon tissues of each group. ELISA kit was used to detect the levels of inflammatory factors. ResultsThe colonic mucosal structure of rats in the UC group was damaged, with glandular loss and infiltration of inflammatory cells. In contrast, rats in the scutellaria baicalensis polysaccharides group had intact colonic mucosal structure and reduced infiltration of inflammatory cells compared with the UC group. Compared with the control group, the expression levels of COX-2, PGE2, Akt protein and mRNA in the colon tissue of UC group rats, as well as the serum levels of inflammatory factors were increased (P＜0.05), while the expression levels of Occludin and ZO-1 protein and mRNA were decreased (P＜0.05). Compared with the UC group, the scutellaria baicalensis polysaccharides group showed a decrease in the expression levels of COX-2, PGE2, Akt protein and mRNA in the colon tissue of rats, as well as a reduction in the levels of inflammatory factors (P＜0.05), while the expression levels of Occludin and ZO-1 protein and mRNA were increased (P＜0.05). ConclusionScutellaria baicalensis polysaccharides may improve the intestinal mucosal barrier function and alleviate inflammatory damage in UC by inhibiting the COX-2/PGE2/Akt signaling pathway. |
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