| 朱风婷,姜靖雯,张慧,陈发章.海南密花核果木枝叶乙醇提取物通过Wnt/β-catenin信号通路抑制肝癌细胞侵袭转移.[J].中南医学科学杂志.,2025,(6):941-946. |
| 海南密花核果木枝叶乙醇提取物通过Wnt/β-catenin信号通路抑制肝癌细胞侵袭转移 |
| EEDC inhibits invasion and metastasis of hepatocellular carcinoma via the Wnt/β-catenin signaling pathway |
| 投稿时间：2024-03-25 修订日期：2025-04-23 |
| DOI：10.15972/j.cnki.43-1509/r.2025.06.001 |
| 中文关键词: 肝癌细胞 Wnt/β-catenin信号通路 EEDC 侵袭转移 |
| 英文关键词:hepatocellular carcinoma cells Wnt/β-catenin signaling pathway EEDC invasion and metastasis |
| 基金项目:海南省自然科学基金青年项目(821QN0995) |
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| 中文摘要: |
| 目的基于Wnt/β-catenin信号通路探讨海南密花核果木枝叶乙醇提取物(EEDC)抑制肝癌细胞侵袭转移的作用机制。 方法将肝癌SMMC-7721细胞分为正常组和1、3、9、27、81 g/L EEDC组；比较各组细胞增殖能力、凋亡率、迁移率、侵袭率、β-catenin、Cyclin D1 mRNA和蛋白表达水平。构建肝癌移植瘤大鼠30只,随机均分为对照组(20 mg/5-FU)、模型组(0.01 mL/g生理盐水)、高EEDC组(2 g/EEDC)、低EEDC组(1 g/EEDC)、联合组(1 g/EEDC+20 mg/5-FU)。比较各组瘤体体积、质量以及β-catenin、Cyclin D1蛋白表达情况。 结果与正常组相比,各EEDC组细胞增殖能力、迁移率、侵袭率、β-catenin、Cyclin D1 mRNA和蛋白表达均降低,且质量浓度越高效果越明显(P＜0.05)。干预24 h后EEDC组细胞凋亡率升高,且质量浓度越高效果越明显(P＜0.05)。与模型组相比,其他组移植瘤体积和质量明显减小,且联合组小于对照组、低EEDC组、高EEDC组(P＜0.05)。与模型组相比,其他组β-catenin、Cyclin D1蛋白表达明显下降,且联合组低于对照组、低EEDC组、高EEDC组(P＜0.05)。 结论EEDC可通过Wnt/β-catenin信号通路抑制肝癌细胞增殖、迁移和侵袭,并诱导癌细胞凋亡。 |
| 英文摘要: |
| AimTo investigate the mechanism by which the ethanol extract of drypetes congestiflora branches and leaves (EEDC) inhibits the invasion and metastasis of hepatocellular carcinoma cells through the Wnt/β-catenin signaling pathway. MethodsHuman hepatocellular carcinoma SMMC-7721 cells were divided into a normal group and EEDC-treated groups (1,3, 9,27, and 81 g/L). Cell proliferation, apoptosis rate, migration rate, invasion rate, and the mRNA and protein expression levels of β-catenin and Cyclin D1 were compared among the groups. A hepatocellular carcinoma xenograft model was established in 30 rats, which were randomly divided into a control group (20 mg/5-FU), a model group (0.01 mL/g normal saline), a high-dose EEDC group (2 g/EEDC), a low-dose EEDC group (1 g/EEDC), and a combination group (1 g/EEDC + 20 mg/5-FU). Tumor volume, tumor mass, and the protein expression levels of β-catenin and Cyclin D1 were compared among the groups. ResultsCompared with the normal group, all EEDC-treated groups showed decreased cell proliferation, migration, invasion, and reduced mRNA and protein expression of β-catenin and Cyclin D1, with the effects being more pronounced at higher concentrations (P＜0.05). After 24 hours of intervention, the apoptosis rate increased in the EEDC-treated groups, also in a concentration-dependent manner (P＜0.05).Compared with the model group, the other groups exhibited significantly reduced tumor volume and mass, with the combination group showing greater reduction than the control, low-dose EEDC, and high-dose EEDC groups (P＜0.05).The protein expression of β-catenin and Cyclin D1 was significantly lower in all treatment groups compared to the model group, and the combination group showed lower expression than the control, low-dose EEDC, and high-dose EEDC groups (P＜0.05). ConclusionEEDC can inhibit the proliferation, migration, and invasion of hepatocellular carcinoma cells and induce apoptosis through the Wnt/β-catenin signaling pathway. |
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