| 刘健,李佳林.上调miR-224-3p对脓毒症大鼠肺损伤及TLR4/NF-κB通路的影响.[J].中南医学科学杂志.,2025,(1):36-39. |
| 上调miR-224-3p对脓毒症大鼠肺损伤及TLR4/NF-κB通路的影响 |
| Effects of upregulation of miR-224-3p on lung injury and TLR4/NF-κB pathway in sepsis rats |
| 投稿时间：2023-03-17 修订日期：2024-03-07 |
| DOI：10.15972/j.cnki.43-1509/r.2025.01.008 |
| 中文关键词: miR-224-3p 脓毒症 肺损伤 大鼠 Toll-样受体4 NF-κB [ |
| 英文关键词:miR-224-3p sepsis lung injury rat TLR4 NF-κB |
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| 中文摘要: |
| 目的探究上调miR-224-3p对脓毒症大鼠肺损伤及Toll-样受体4(TLR4)/核因子-κB(NF-κB)通路的影响。 方法将64只SD大鼠随机均分为假手术组、模型组、miR-224-3p模拟物(mimics)组、mimics-control组。采用盲肠结扎穿孔术建立脓毒症肺损伤大鼠模型。ELISA检测各组大鼠血清炎症因子水平；测量肺组织湿/干质量(W/D)比值评估肺水肿的严重程度；HE染色观察各组肺组织病理变化；qRT-PCR检测各组肺组织miR-224-3p表达水平；Western blotting检测肺组织TLR4/NF-κB通路相关蛋白表达情况。 结果模型组、mimics-control组大鼠肺组织病理损伤严重,大量炎症细胞浸润；miR-224-3p mimics组大鼠肺组织病理损伤明显减轻,少量炎症细胞浸润。与假手术组比较,其他各组大鼠肺组织miR-224-3p表达降低,血清炎症因子水平、肺组织W/D比值、TLR4/NF-κB通路相关蛋白表达均升高(P＜0.05)。与模型组或mimics-control组比较,miR-224-3p mimics组大鼠肺组织miR-224-3p表达升高,血清炎症因子水平、肺组织W/D比值、TLR4/NF-κB通路相关蛋白表达均降低(P＜0.05)。 结论上调miR-224-3p可减轻脓毒症大鼠肺损伤,其作用机制可能与抑制TLR4/NF-κB信号通路的活化有关。 |
| 英文摘要: |
| AimTo investigate the effects of upregulating miR-224-3p on lung injury and Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) pathway in sepsis rats. MethodsSixty-four SD rats were randomly divided into sham surgery group, model group, miR-224-3p mimics group and mimics-control group. The rat model of sepsis-induced lung injury was established by cecal ligation and perforation . The levels of inflammatory factors were detected by ELISA. The wet/dry (W/D) weight ratio of lung tissue was measured to assess the severity of pulmonary edema. The pathological changes in lung tissue of each group was observed by HE staining. The expression of miR-224-3p in lung tissue was detected by qRT-PCR. The expression of TLR4/NF-κB pathway related proteins in lung tissue was detected by Western blotting. ResultsThe pathological damage to the lung tissue of rats in the model group and the mimics-control group was severe, accompanying with a large number of inflammatory cell infiltration. However, the pathological damage to the lung tissue of rats in the miR-224-3p mimics group was significantly reduced, with only a small amount of inflammatory cell infiltration. Compared with the sham surgery group, the expression of miR-224-3p in the lung tissue of rats in other groups were decreased, while the levels of serum inflammatory factors, W/D ratio in lung tissue, and expression of TLR4/NF-κB pathway related proteins were increased (P＜0.05). Compared with the model group or mimics-control group, the above indicators of rats were reversed in the miR-224-3p mimics group (P＜0.05). ConclusionUpregulation of miR-224-3p can reduce lung injury in sepsis rats, and its mechanism may be related to the inhibition of TLR4/NF-κB signaling pathway activation. |
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