| 朱敬儒,白辰,王洋,卓丽清,苏晨,孙炜喆,王旭堃,刘铁钢.基于生物信息学及动物实验探究糖酵解与肺炎的关系.[J].中南医学科学杂志.,2025,(1):7-11. |
| 基于生物信息学及动物实验探究糖酵解与肺炎的关系 |
| Exploration the relationship between glycolysis and pneumonia based on bioinformatics and animal experiments |
| 投稿时间：2023-12-21 修订日期：2024-10-03 |
| DOI：10.15972/j.cnki.43-1509/r.2025.01.002 |
| 中文关键词: 糖酵解 肺炎 生物信息学 动物实验 [ |
| 英文关键词:glycolysis pneumonia bioinformatics animal experiment |
| 基金项目:国家自然科学基金(82104567)；国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-C-202006)；北京中医药大学揭榜挂帅课题(2022-JYB-JBZR-030) |
| 作者 | 单位 | E-mail | | 朱敬儒 | 北京中医药大学中医学院,北京 100029 | e-mail为zhujingru2020@163.com,e-mail为liutiegang2009@163.com | | 白辰 | 北京中医药大学中医学院,北京 100029 | | | 王洋 | 北京中医药大学中医学院,北京 100029 | | | 卓丽清 | 北京中医药大学中医学院,北京 100029 | | | 苏晨 | 北京中医药大学中医学院,北京 100029 | | | 孙炜喆 | 北京中医药大学中医学院,北京 100029 | | | 王旭堃 | 北京中医药大学中医学院,北京 100029 | | | 刘铁钢 | 北京中医药大学中医学院,北京 100029 | e-mail为zhujingru2020@163.com,e-mail为liutiegang2009@163.com |
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| 中文摘要: |
| 目的通过生物信息学和动物实验探究糖酵解与肺炎的关系。 方法通过GEO数据库GSE40012数据集,选取62例肺炎患者(肺炎组)和35例健康者(健康组)的全血样本,比较两组糖酵解关键酶烯醇化酶1(ENO1)、M2型丙酮酸激酶(PKM2)蛋白及抑炎因子白细胞介素(IL)-10表达水平；采用Spearman分析PKM2与IL-10的相关性,富集分析差异表达基因的生物学通路。将16只雄性SPF级SD大鼠均分为正常组(纯水雾化)和模型组(脂多糖雾化吸入)；采用HE染色和PAS染色分别观察两组大鼠肺组织病理、糖原变化；采用ELISA法检测大鼠肺组织炎症因子肿瘤坏死因子-α(TNF-α)、IL-1β、IL-10水平；Western blotting检测肺组织中促炎巨噬细胞的主要标志物诱导型一氧化氮合酶(iNOS)、缺氧诱导因子(HIF-1α)、ENO1、PKM2蛋白水平。 结果与健康组比较,肺炎组ENO1、PKM2表达上调(P＜0.05)；IL-10表达下调,且与PKM2表达呈负相关(P＜0.05)。肺炎患者能量代谢相关通路的生物学进程为氧化磷酸化、线粒体ATP合成、ATP合成耦合电子传递、ATP代谢过程等。动物实验结果显示,与正常组比较,模型组大鼠肺组织iNOS、TNF-α、IL-1β、HIF-1α、ENO1、PKM2水平升高(P＜0.05),IL-10降低(P＜0.05)。 结论在肺炎过程中,肺组织糖酵解增多,可能与通过HIF-1α、ENO1、PKM2蛋白的高表达参与炎症过程有关。 |
| 英文摘要: |
| AimTo explore the relationship between glycolysis and pneumonia by bioinformatics and animal experiments. MethodsThe whole blood samples of 62 patients with pneumonia (pneumonia group) and 35 healthy people (health group) were selected from the GSE40012 data set of the GEO database. The expression levels of key enzyme glyase enzyme enolase 1 (ENO1), pyruvate kinase M2 (PKM2) protein and anti-inflammatory factor interleukin (IL)-10 of the two groups were compared. Spearman analysis was used to investigate the correlation between PKM2 and IL-10. Biological pathways of differentially expressed genes was analyzed by using enrichment analysis. Sixteen male SPF-grade SD rats were divided into normal group (pure water atomization) and model group (lipopolysaccharide atomization inhalation). HE staining and PAS staining were used to observe the pathological and glycogen changes in the lung tissues of rats of two groups, respectively. ELISA was used to detect the levels of inflammatory factors tumor necrosis factor-α (TNF-α), IL-1β, and IL-10 in rat lung tissue. Western blotting was used to detect the levels of the main markers inducible nitric oxide synthase (iNOS), hypoxia inducible factor-1 α (HIF-1 α), ENO1, and PKM2 proteins. ResultsCompared with the healthy group, the expression of ENO1 and PKM2 in the pneumonia group were increased (P＜0.05). The expression of IL-10 was decreased and was negatively correlated with the gene expression of the PKM2 (P＜0.05). The biological processes of energy metabolism-related pathways in pneumonia patients were oxidative phosphorylation, mitochondrial ATP synthesis, ATP synthesis coupled electron transfer, and ATP metabolism process. The animal experiment results showed that compared with the normal group, the levels of iNOS, TNF-α, IL-1β, HIF-1α, ENO1, and PKM2 of the lung tissue of rats in the model group were increased (P＜0.05), while IL-10 was decreased (P＜0.05). ConclusionThe increase of lung glycolysis in the course of pneumonia may be related with the involvement of inflammatory process through the high expression of HIF-1α, ENO1 and PKM2 proteins. |
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