| 孙婷,杨毅,董俊飞,崔兴华.感染性心内膜炎患者金黄色葡萄球菌耐药基因及毒力基因分析.[J].中南医学科学杂志.,2024,(6):1006-1009. |
| 感染性心内膜炎患者金黄色葡萄球菌耐药基因及毒力基因分析 |
| Analysis of drug resistance and virulence genes of Staphylococcus aureus in patients with infective endocarditis |
| 投稿时间：2024-01-30 修订日期：2024-10-29 |
| DOI：10.15972/j.cnki.43-1509/r.2024.06.033 |
| 中文关键词: 感染性心内膜炎 金黄色葡萄球菌 耐药基因 毒力基因 |
| 英文关键词:infective endocarditis Staphylococcus aureus drug resistance gene virulence gene |
| 基金项目:沈阳市卫生健康委科研项目(2023WS0153) 作者简介:孙婷,硕士,主治医师,研究方向为感染性疾病的诊治,E-mail为grew0102@163.com。 |
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| 中文摘要: |
| 目的调查感染性心内膜炎(IE)患者金黄色葡萄球菌(SA)分离株的耐药基因、毒力基因携带情况。 方法收集确诊IE的82例患者血标本,进行细菌鉴定及药敏实验,提取SA菌株DNA后,采用聚合酶链反应进行耐药基因和毒力基因筛查,分析SA感染的危险因素。 结果82份标本共95株病原菌菌株,鉴定出SA菌株22株(23.16%),红霉素等9种药物对SA耐药。耐药基因共检出vang(45.45%)、tetL(40.91%)、vana(36.36%)、vane(31.82%)、aph(22.73%)等16种SA抗生素耐药基因。22株SA菌株中肠毒素、溶血性毒素、杀白细胞毒素及黏附毒素均有检出,其中以肠毒素中sea基因和溶血性毒素中hlb基因检出率较高(54.54%)。Logistic回归分析显示,年龄、栓塞、手术及中心静脉置管是SA感染的危险因素(P＜0.05)。 结论IE患者的SA检出率、耐药基因及毒力基因携带率均较高,年龄、栓塞、手术及中心静脉置管均可能影响SA感染。 |
| 英文摘要: |
| AimTo investigate drug resistance and virulence genes of Staphylococcus aureus (SA) strains isolated from patients with infective endocarditis (IE). MethodsCollect blood samples from 82 patients diagnosed with IE, conduct bacterial identification and drug sensitivity tests, extract SA strain DNA, and screen for resistance and virulence genes using polymerase chain reaction to analyze risk factors for SA infection. ResultsA total of 95 pathogenic bacteria strains were isolated from 82 specimens, and 22 (23.16%) SA strains were identified. SA was resistant to nine kinds of drugs such as erythromycin. A total of 16 kinds of antibiotic resistance genes in SA were detected, including vang (45.45%), tetL (40.91%), vana (36.36%), vane (31.82%), aph (22.73%), etc. Enterotoxin, hemolytic toxin, panton-valentine leukocidin and adhesion toxin were detected in all of the 22 SA strains. The detection rates of sea in enterotoxin and hlb in hemolytic toxin were the highest (54.54%). Logistic regression analysis showed that age, embolism, surgery and central venous catheterization were risk factors for SA infection (P＜0.05). ConclusionThe detection rate of SA and carrying rates of drug resistance and virulence genes are high in patients with IE. Age, embolism, surgery and central venous catheterization are risk factors for SA infection. |
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