| 向健,王晶,王俊,田文武.PCR-SSP技术联合抗人球蛋白卡式法在ABO和RhD血型鉴定困难患者中的应用.[J].中南医学科学杂志.,2024,(6):972-975. |
| PCR-SSP技术联合抗人球蛋白卡式法在ABO和RhD血型鉴定困难患者中的应用 |
| Application of PCR-SSP combined with anti-human globulin cassette method in patients with difficulty in ABO/RhD blood type identification |
| 投稿时间：2023-11-07 修订日期：2024-02-05 |
| DOI：10.15972/j.cnki.43-1509/r.2024.06.024 |
| 中文关键词: PCR-SSP技术 抗人球蛋白卡式法 ABO血型 RhD血型 血型鉴定困难 |
| 英文关键词:PCR-SSP anti-human globulin cassette method ABO blood type RhD blood type difficulty in blood type identification |
| 基金项目:湖北省卫生健康委员会联合基金项目(WJ2019H549) 作者简介:向健,副主任技师,研究方向为输血医学及医学检验,E-mail为86226758@qq.com。 |
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| 中文摘要: |
| 目的探究聚合酶链反应-序列特异性引物(PCR-SSP)技术联合抗人球蛋白卡式法在ABO和RhD血型鉴定困难患者中的应用。 方法选取118例ABO与RhD血型鉴定困难患者血型标本,分别应用PCR-SSP技术、抗人球蛋白卡式法分析患者血型情况。 结果118例血型鉴定困难者采用抗人球蛋白卡式法联合PCR-SSP技术明确检出ABO血型113例,同时应用PCR-SSP可以确定ABO亚型,5例未检出血型的样品经PCR-SSP测序发现,基因分型分别为BO02、A205O01、A102B01、A201B01、B01B01,分别出现第7外显子640A＞G杂合突变、第7外显子1009A＞G杂合突变、第3外显子150G＞C杂合突变、第6外显子240T＞C、第7外显子431G＞T杂合突变；采用抗人球蛋白卡式法联合PCR-SSP检出RhD血型114例,未检出的4例进行测序,1例显示为RhD weak D 779A/G突变；3例为RhD weak D type 4.0(包括602C/G、667T/G以及819G/A突变)。 结论ABO和RhD血型鉴定困难患者应用PCR-SSP技术联合抗人球蛋白卡式法进行鉴定可以准确鉴定血型,并发现血型具体亚型,保证输血安全。 |
| 英文摘要: |
| AimTo explore the application of polymerase reaction sequence specific primers (PCR-SSP) technology combined with anti human globulin cartridge assay in patients with difficulty in ABO and RhD blood type identification. MethodsBlood samples of 118 patients with difficulty in ABO/RhD blood type identification were collected for blood typing by PCR-SSP and anti-human globulin cassette method. ResultsFor the 118 patients, anti-human globulin cassette method and PCR-SSP confirmed that there were 113 patients with ABO type. The ABO subtypes were determined by PCR-SSP. For 5 undetermined samples, PCR-SSP sequencing found the genotypes were BO02, A205O01, A102B01, A201B01 and B01B01, which exhibited the 7th exon 640A＞G heterozygous mutation, the 7th exon 1009A＞G heterozygous mutation, the 3rd exon 150G＞C heterozygous mutation, the 6 exon 240T＞C, and the 7th exon 431G＞T heterozygous mutation. Anti-human globulin cassette method combined with PCR-SSP detected 114 cases of RhD type, and 4 cases not detected were subjected to sequencing. 1 case showed RhD weak D 779A/G mutation, and 3 cases showed RhD weak D type 4.0 (including 602C/G, 667T/G and 819G/A mutations). ConclusionThe application of PCR-SSP combined with anti-human globulin cassette method in patients with difficulty in ABO/RhD blood type identification can help identify blood types accurately, and find out specific subtypes of blood types, thus improving the safety of blood transfusion. |
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