| 高丽雯,郑重,陆心仪,丁君,张立东,何静.瑞马唑仑通过Akt/mTOR信号通路对CIRI大鼠脑神经元的保护作用.[J].中南医学科学杂志.,2024,(6):902-905, 914. |
| 瑞马唑仑通过Akt/mTOR信号通路对CIRI大鼠脑神经元的保护作用 |
| The protective effects of remifentanil on brain neurons in rats with cerebral ischemia reperfusion injury through the Akt/mTOR signaling pathway |
| 投稿时间：2024-08-27 修订日期：2024-10-30 |
| DOI：10.15972/j.cnki.43-1509/r.2024.06.006 |
| 中文关键词: 瑞马唑仑 CIRI 神经元保护 自噬 Akt/mTOR |
| 英文关键词:remimazolam cerebral ischemia reperfusion injury neuron protection autophagy Akt/mTOR |
| 基金项目:太仓市基础研究计划及重点研发计划(社会发展)项目(TC2023JCYLD03)；吴阶平医学基金会临床科研专项资助基金(320.6750.2024-15-9) 作者简介:高丽雯,硕士,副主任医师,研究方向为手术麻醉,E-mail为aewp20220511@163.com。通信作者何静,副主任医师,研究方向为老年疾病麻醉、骨科麻醉,E-mail为15995582577@163.com。 |
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| 中文摘要: |
| 目的探究瑞马唑仑通过蛋白激酶B/雷帕霉素靶蛋白(Akt/mTOR)信号通路对脑缺血再灌注损伤(CIRI)大鼠脑神经元的影响。 方法将32只SD大鼠随机均分为假手术组(不插入线栓)、模型组、瑞马唑仑组和NVP-BEZ235(信号通路抑制剂)组,除假手术组外其他组均采用线栓法建立CIRI大鼠模型。采用Longa评分评估大鼠神经功能,干湿重法检测脑水肿程度,TTC染色法测定大鼠脑梗死体积,ELISA法检测脑组织炎症因子、氧化应激指标,蛋白印迹法检测脑组织自噬标志物及Akt/mTOR信号通路表达水平。 结果与模型组比较,瑞马唑仑组Longa评分、脑含水率、脑梗死体积比、脑组织白细胞介素(IL)-6、IL-1β、肿瘤坏死因子-α、丙二醛含量、微管相关蛋白1轻链3-Ⅱ/Ⅰ(LC3-Ⅱ/Ⅰ)、自噬相关基因(Beclin-1)表达降低,超氧化物歧化酶、谷胱甘肽含量、p-Akt/Akt、p-mTOR/mTOR水平升高(P＜0.05)；而Akt/mTOR信号通路抑制剂NVP-BEZ235可部分减弱瑞马唑仑的作用。 结论瑞马唑仑可能通过激活Akt/mTOR信号通路,从而在CIRI大鼠中发挥脑神经元保护作用。 |
| 英文摘要: |
| AimTo explore the effects of remifentanil on brain neurons in rats with cerebral ischemia reperfusion injury (CIRI) through the protein kinase B/rapamycin target protein (Akt/mTOR) signaling pathway. Methods32 SD rats were randomly divided into sham operation group (without inserting a suture), model group, remifentanil group, and NVP-BEZ235 (signaling pathway inhibitor) group. Except for the sham surgery group, all other groups were established as CIRI rat models using the suture method. Longa score was used to evaluate the neurological function of rats, dry wet weight method was used to detect the degree of brain edema, TTC staining method was used to determine the volume of cerebral infarction in rats, ELISA method was used to detect inflammatory factors and oxidative stress indicators in brain tissue, and Western blotting method was used to detect the expression levels of autophagy markers and Akt/mTOR signaling pathway in brain tissue. ResultsCompared with the sham surgery group, the model group showed an increase in Longa score, brain water content, cerebral infarction volume ratio, interleukin (IL)-6, IL-1β, tumor necrosis factor-α, malondialdehyde, microtubule associated protein 1 light chain 3-Ⅱ/Ⅰ, autophagy related genes, and a decrease in superoxide dismutase, glutathione, phosphorylation Akt/Akt, and p-mTOR/mTOR (P＜0.05). Compared with the model group, the remifentanil group reversed the above trend (P＜0.05). The Akt/mTOR signaling pathway inhibitor NVP-BEZ235 can partially attenuate the effects of remifentanil (P＜0.05). ConclusionRimazolen may exert neuroprotective effects on brain neurons in CIRI rats by activating the Akt/mTOR signaling pathway. |
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