| 李东阳,李沐阳,孙学胜.LncRNA-MALAT1/miR-200b-3p/GLI3轴调控胆管癌细胞HCCC9810增殖、侵袭和上皮间质转化.[J].中南医学科学杂志.,2024,(6):882-886. |
| LncRNA-MALAT1/miR-200b-3p/GLI3轴调控胆管癌细胞HCCC9810增殖、侵袭和上皮间质转化 |
| lncRNA-MALAT1/miR-200b-3p/GLI3 axis regulates the proliferation, invasion and epithelial interstitial transformation of cholangiocarcinoma cell line HCCC9810 |
| 投稿时间：2023-08-08 修订日期：2023-12-15 |
| DOI：10.15972/j.cnki.43-1509/r.2024.06.002 |
| 中文关键词: 胆管癌 lncRNA-MALAT1 miR-200b-3p GLI3 上皮间质转化 |
| 英文关键词:cholangiocarcinoma lncRNA-MALAT1 miR-200b-3p GLI3 epithelial interstitial transformation |
| 基金项目:河北省卫生和计划生育委员会科研基金项目(20230260) 作者简介:李东阳,主治医师,研究方向为肝胆胃肠常见疾病的诊治,E-mail为homewok168@163.com。 |
|
| 摘要点击次数: 80 |
| 全文下载次数: 28 |
| 中文摘要: |
| 目的探讨长链非编码RNA(lncRNA)-MALAT1/miR-200b-3p/GLI3轴对胆管癌细胞HCCC9810增殖、侵袭和上皮间质转化的影响。 方法采用qRT-PCR检测胆管癌组织及细胞中lncRNA-MALAT1、miR-200b-3p、GLI3 mRNA表达水平；采用CCK-8、平板克隆实验、Transwell实验分别检测细胞增殖、克隆形成及细胞侵袭能力；验证miR-200b-3p与lncRNA-MALAT1及GLI3的关系；Western blotting检测上皮间质转化相关蛋白。 结果胆管癌组织中lncRNA-MALAT1和GLI3表达高于癌旁组织,miR-200b-3p表达低于癌旁组织(P＜0.05)；各胆管癌细胞系中lncRNA-MALAT1和GLI3表达高于正常胆管上皮HIBEpiC细胞系,miR-200b-3p表达低于正常胆管上皮HIBEpiC细胞系(P＜0.05)。si-MALAT1组细胞增殖能力、克隆形成能力、侵袭能力和N-cadherin、Vimentin、Snail蛋白表达低于si-NC组(P＜0.05)；si-MALAT1组细胞E-cadherin蛋白高于si-NC组(P＜0.05)；miR-200b-3p mimics可降低MALAT1野生型和GLI3野生型的荧光素酶活性(P＜0.05)；si-MALAT1+miR-200b-3p inhibitor组和si-MALAT1+GLI3组细胞增殖能力、克隆形成能力、侵袭能力和N-cadherin、Vimentin、Snail蛋白表达都高于si-MALAT1组(P＜0.05)；si-MALAT1+miR-200b-3p inhibitor组和si-MALAT1+GLI3组的细胞E-cadherin蛋白表达低于si-MALAT1组(P＜0.05)。 结论lncRNA-MALAT1在胆管癌组织和细胞中高表达,干扰MALAT1可以通过调控miR-200b-3p/GLI3分子轴抑制胆管癌细胞HCCC9810增殖、侵袭及上皮间质转化。 |
| 英文摘要: |
| AimTo investigate the regulation of the proliferation, invasion and epithelial interstitial transformation of cholangiocarcinoma cell line HCCC9810 by the long non-coding RNA (lncRNA) MALAT1/miR-200b-3p/GLI3 axis. MethodsExpression levels of lncRNA-MALAT1, miR-200b-3p and GLI3 mRNA in tissues and cells were detected by qRT-PCR. Cell proliferation, clonogenesis and cell invasion were detected. The relationship between lncRNA-MALAT1 and miR-200b-3p and GLI3 was verified. The proteins related to epithelial interstitial transformation were detected by Western blotting. ResultsThe expressions of lncRNA-MALAT1 and GLI3 in cholangiocarcinoma were noteworthy higher than those in paracancer tissues, and the expressions of miR-200b-3p in cholangiocarcinoma tissues were noteworthy lower (P＜0.05). The expressions of lncRNA-MALAT1 and GLI3 in various cell lines of cholangiocarcinoma were markedly higher than those in normal HIBEpiC cell lines (P＜0.05). The expression of miR-200b-3p was visible lower than that in normal bile duct epithelial HIBEpiC cells (P＜0.001). The ability of cell proliferation, clonogenesis, invasion and N-cadherin, Vimentin, Snail proteins expression in si-MALAT1 group was obviously lower than that in si-NC group (P＜0.05). The E-cadherin proteins in si-MALAT1 group were visible higher than those in si-NC group (P＜0.05). miR-200b-3p mimics noteworthy decreased the luciferase activities of MALAT1-wt and GLI3-wt (P＜0.05). Cell proliferation ability, clonogenesis ability, invasion ability and N-cadherin, Vimentin, Snail proteins expression of si-MALAT1+miR-200b-3p inhibitor group and si-MALAT1+GLI3 inhibitor group were noteworthy higher than those of si-MALAT1 group (P＜0.05). The E-cadherin protein expression of si-MALAT1+miR-200b-3p inhibitor group and si-MALAT1+GLI3 inhibitor group was noteworthy lower than that of si-MALAT1 group (P＜0.05). ConclusionlncRNA-MALAT1 is highly expressed in bile duct carcinoma tissues and cells, and interference with MALAT1 can inhibit proliferation, invasion and epithelial mesenchymal transformation of bile duct cancer cells HCCC9810 by regulating the molecular axis of miR-200b-3p/GLI3. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|