| 黄海霞,吴芷莹,王秋平,曾利,彭诗慧,刘慧清,楼萍,柏琴琴.噬菌体LLY联合左氧氟沙星对泛耐药鲍曼不动杆菌ABLL生物被膜的抑制和清除作用.[J].中南医学科学杂志.,2024,(5):715-720. |
| 噬菌体LLY联合左氧氟沙星对泛耐药鲍曼不动杆菌ABLL生物被膜的抑制和清除作用 |
| Inhibition and clearance effects of bacteriophage LLY combined with levofloxacin on the biofilm of extensively drug-resistant Acinetobacter baumannii ABLL |
| 投稿时间：2024-04-15 修订日期：2024-06-06 |
| DOI：10.15972/j.cnki.43-1509/r.2024.05.006 |
| 中文关键词: 噬菌体LLY 左氧氟沙星 生物被膜 泛耐药鲍曼不动杆菌ABLL 联合作用 |
| 英文关键词:bacteriophage LLY LEV biofilm extensively drug-resistant Acinetobacter baumannii ABLL combined effect |
| 基金项目:湖南省自然科学基金(2023JJ30501);湖南省教育厅科学研究项目(21B0399) |
| 作者 | 单位 | E-mail | | 黄海霞 | 南华大学衡阳医学院 公共卫生学院卫生检验与检疫系,湖南衡阳 421001 | e-mail为2585516186@qq.com,e-mail为baiqinqin1213@126.com | | 吴芷莹 | 南华大学衡阳医学院 公共卫生学院卫生检验与检疫系,湖南衡阳 421001 | | | 王秋平 | 南华大学衡阳医学院 附属第一医院检验医学中心,湖南衡阳 421001 | | | 曾利 | 衡阳市中医医院,湖南衡阳 421001 | | | 彭诗慧 | 南华大学衡阳医学院 公共卫生学院卫生检验与检疫系,湖南衡阳 421001 | | | 刘慧清 | 南华大学衡阳医学院 公共卫生学院卫生检验与检疫系,湖南衡阳 421001 | | | 楼萍 | 衡阳市疾病预防控制中心,湖南衡阳 421001 | | | 柏琴琴 | 南华大学衡阳医学院 公共卫生学院卫生检验与检疫系,湖南衡阳 421001 | e-mail为2585516186@qq.com,e-mail为baiqinqin1213@126.com |
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| 中文摘要: |
| 目的探究噬菌体LLY联合左氧氟沙星(LEV)对泛耐药鲍曼不动杆菌ABLL生物被膜的作用。 方法生物被膜实验分为不同浓度LLY组、不同质量浓度LEV组、不同浓度LLY与不同质量浓度LEV联合组、对照组(不处理)。采用结晶紫染色法检测生物被膜的生物量,MTT法检测生物被膜细胞相对活性,显微成像技术观察生物被膜结构。利用双层琼脂平板法测定LEV处理后的噬菌斑直径；点滴裂解实验检测LEV诱导鲍曼不动杆菌ABLL释放前噬菌体的情况。 结果与对照组比较,106 PFU/mL LLY+8 mg/L LEV组的ABLL生物被膜的形成量和细胞相对活性降低(P＜0.05),且降低效果优于8 mg/L LEV组和106 PFU/mL LLY组(P＜0.05),该联合组对生物被膜网状结构破坏效果尤为明显。与对照组相比,不同质量浓度LEV处理组噬菌斑的直径增大(P＜0.05)。LEV可以诱导ABLL释放前噬菌体。 结论噬菌体LLY联合LEV可以有效抑制或清除泛耐药鲍曼不动杆菌ABLL的生物被膜。 |
| 英文摘要: |
| AimTo explore the effects of bacteriophage LLY combined with levofloxacin (LEV) on the control of extensively drug-resistant Acinetobacter baumannii ABLL biofilm. MethodsThe biofilm experiment was divided into different concentrations of LLY groups, different mass concentrations of LEV groups, different concentrations of LLY combined with different mass concentrations of LEV groups, and control group. Crystal violet staining method was used to detect the biomass of biofilm. MTT method was used to detect the viability of biofilm cells. The structure of biofilm was observed by microscopic imaging method. The diameter of plaque under LEV was measured by the double layer agar plate method. ABLL-induced prophage release carried by LEV was detected by spot lysis test. ResultsCompared with the control group, the combination of 106 PFU/mL LLY+8 mg/L LEV group showed a decrease in the ABLL biofilm biomass and the viability of biofilm bacteria (P＜0.05), and the reduction effect was better than that of the 8 mg/L LEV group and the 106 PFU/mL LLY group (P＜0.05). Furthermore, the combination group showed significant disruption of the reticular structure of the biofilm. The diameter of the phage plaques was increased in the LEV group compared with the group without LEV (P＜0.05). LEV could induce the release of prophages from ABLL. ConclusionThe combination of bacteriophage LLY and LEV could effectively prevent or eradicate the biofilm of extensively drug-resistant Acinetobacter baumannii ABLL. |
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