| 姜媛娟,郑水林,杨飞.微囊藻毒素-LR染毒对小鼠肝脏糖脂代谢的影响.[J].中南医学科学杂志.,2024,(5):695-699. |
| 微囊藻毒素-LR染毒对小鼠肝脏糖脂代谢的影响 |
| Effects of microcystin-LR on liver glucose and lipid metabolism in mice |
| 投稿时间：2024-06-02 修订日期：2024-08-07 |
| DOI：10.15972/j.cnki.43-1509/r.2024.05.002 |
| 中文关键词: 微囊藻毒素-LR 肝脏 糖脂代谢 小鼠 |
| 英文关键词:MC-LR liver glycolipid metabolism mice |
| 基金项目:国家自然科学基金(81773393)；湖南省重点研发项目(2022SK2089) |
| 作者 | 单位 | E-mail | | 姜媛娟 | 南华大学衡阳医学院公共卫生学院,湖南衡阳 4210010 [专家简介]〖TP24-05J杨飞.tif
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Z1,Y#〗 杨飞,博士,教授,博士研究生导师,湖南省青年骨干教师、湖南省优青获得者、生态健康与人类重要疾病防控湖南省高校重点实验室副主任、水环境与农产品安全和典型环境污染与健康危害湖南省重点实验室骨干成员、南华大学高层次人才学科拔尖人才 | e-mail为3330945289@qq.com,e-mail为phfyang@usc.edu.com | | 郑水林 | 南华大学衡阳医学院公共卫生学院,湖南衡阳 4210010 [专家简介]〖TP24-05J杨飞.tif
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Z1,Y#〗 杨飞,博士,教授,博士研究生导师,湖南省青年骨干教师、湖南省优青获得者、生态健康与人类重要疾病防控湖南省高校重点实验室副主任、水环境与农产品安全和典型环境污染与健康危害湖南省重点实验室骨干成员、南华大学高层次人才学科拔尖人才 | | | 杨飞 | 南华大学衡阳医学院公共卫生学院,湖南衡阳 4210010 [专家简介]〖TP24-05J杨飞.tif
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Z1,Y#〗 杨飞,博士,教授,博士研究生导师,湖南省青年骨干教师、湖南省优青获得者、生态健康与人类重要疾病防控湖南省高校重点实验室副主任、水环境与农产品安全和典型环境污染与健康危害湖南省重点实验室骨干成员、南华大学高层次人才学科拔尖人才 | e-mail为3330945289@qq.com,e-mail为phfyang@usc.edu.com |
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| 中文摘要: |
| 目的探讨微囊藻毒素-LR(MC-LR)对小鼠肝脏糖脂代谢的影响。 方法将40只雄性C57BL/6小鼠随机均分成对照组、低染毒组、中染毒组、高染毒组,各组分别给与含0、1、60、120 μg/L MC-LR的饮用水染毒6个月。除对照组外,其他各组构建MC-LR慢性染毒体内模型。HE、油红O染色观察各组小鼠肝脏组织病理学改变情况。ELISA检测各组小鼠血清白脂素、胰岛素(INS)水平。比较各组小鼠肝脏质量、肝脏指数和血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、血糖、甘油三酯(TG)、总胆固醇(TC)、游离脂肪酸(FFA)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇水平。 结果各染毒组小鼠肝脏体质量及肝脏指数与对照组比较,差异均无显著性(P＞0.05)。HE、油红O染色结果显示,对照组、低染毒组小鼠肝小叶结构完整,肝细胞排列有序,未见明显病理改变；中、高染毒组小鼠肝小叶排列紊乱,可见少量炎症细胞浸润及脂肪变性,且高染毒组脂肪变性程度最为严重。与对照组相比,中、高染毒组GPT、GOT、TG、TC、FFA、白脂素、INS和血糖水平上升(P＜0.05),HDLC水平下降(P＜0.05)。 结论MC-LR染毒处理可诱导小鼠肝脏糖脂代谢紊乱。 |
| 英文摘要: |
| AimTo investigate the effects of microcystin-LR (MC-LR) on liver glucose and lipid metabolism in mice. MethodsA total of 40 male C57BL/6 mice were randomly divided into control group, low toxicity group, medium toxicity group and high toxicity group, and were given drinking water containing 0,1, 60 and 120 μg/L MC-LR for 6 months, respectively. Except the control group, the in vivo model of chronic MC-LR infection were constructed in other groups. The histopathological changes of liver were observed by HE and oil red O staining. Levels of serum asprosin and insulin (INS) were detected by ELISA kits. The liver weight, liver index and serum levels of glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood sugar, triglyceride (TG), total cholesterol (TC), free fatty acid (FFA), high density lipoprotein cholesterol (HDLC) and low density lipoprotein cholesterol were compared among all groups. ResultsThere was no significant difference in liver body mass and liver coefficient between the poisoned groups of mice and the control group (P＞0.05). The results of HE and oil red O staining showed that the liver lobule structure of mice of control group and low toxicity group was contact, liver cells were arranged in normal order, and no obvious pathological changes were observed. Hepatic lobule arrangement was disordered in the medium and high toxicity groups, with a small amount of inflammatory cell infiltration and steatosis observed, and the degree of steatosis was the most serious in the high toxicity group. Compared with the control group, the levels of GPT, GOT, TG, TC, FFA, asprosin, INS and blood sugar in medium and high toxicity groups were increased (P＜0.05), while the levels of HDLC were decreased (P＜0.05). ConclusionThe exposure of MC-LR can induce disorder of liver glucose and lipid metabolism in mice. |
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