| 丁珍珍,王丽丽,闫锡钊,安凤兰,杨晓晖.宫颈病变患者病灶组织miR-21、miR-146a、miR-224表达与HR-HPV感染、宫颈病变程度的关系.[J].中南医学科学杂志.,2024,(4):661-664. |
| 宫颈病变患者病灶组织miR-21、miR-146a、miR-224表达与HR-HPV感染、宫颈病变程度的关系 |
| Association between the expression of miR-21, miR-146a and miR-224 in lesion tissues of patients with cervical lesions and HR-HPV infection and the degree of cervical lesions |
| 投稿时间：2023-10-12 修订日期：2024-05-20 |
| DOI：10.15972/j.cnki.43-1509/r.2024.04.040 |
| 中文关键词: HR-HPV 宫颈病变 miR-21 miR-146a miR-224 |
| 英文关键词:HR-HPV cervical lesion miR-21 miR-146a miR-224 |
| 基金项目: |
|
| 摘要点击次数: 153 |
| 全文下载次数: 160 |
| 中文摘要: |
| 目的分析宫颈病变患者病灶组织miR-21、miR-146a、miR-224表达与高危型人乳头状瘤病毒(HR-HPV)感染、宫颈病变程度的关系。 方法选取宫颈病变患者252例,根据宫颈病变程度分为宫颈癌组、低级别鳞状上皮内病变(LSIL)组、高级别鳞状上皮内病变(HSIL)组；根据是否感染HR-HPV分为感染组和非感染组。另选宫颈正常的子宫肌瘤患者84例为对照组。PCR检测各组miR-21、miR-146a和miR-224表达水平,并分析宫颈癌患者临床病理特征与miR-21、miR-146a和miR-224表达的关系。 结果感染组、非感染组miR-224水平高于对照组,感染组miR-224水平高于非感染组,宫颈癌组高于对照组、LSIL组、HSIL组(P＜0.05)。低分化、FIGO分期Ⅱb~Ⅲa期、淋巴结转移、HR-HPV感染的宫颈癌患者病灶组织中miR-224高于中高分化、FIGO分期Ⅰb~Ⅱa期、无淋巴结转移及非HR-HPV感染者(P＜0.05)。分化程度、HR-HPV感染、FIGO分期、淋巴结转移是影响宫颈病变患者病灶组织miR-224水平的影响因素。 结论宫颈癌组织miR-224表达明显上调,且HR-HPV感染者miR-224表达水平更高；HR-HPV感染、分化程度、FIGO分期、淋巴结转移与宫颈癌组织miR-224表达密切相关。 |
| 英文摘要: |
| AimTo analyze the association between the expression of miR-21, miR-146a and miR-224 in lesion tissues of patients with cervical lesions and high risk-human papilloma virus (HR-HPV) infection and the degree of cervical lesions. MethodsAccording to the degree of cervical lesions, 252 patients were divided into the cervical cancer group, the low-grade squamous intraepithelial lesion (LSIL) group and the high-grade squamous intraepithelial lesion (HSIL) group. According to the presence or absence of HR-HPV infection, the patients were divided into infection group and non-infection group. Meanwhile, 84 patients with normal cervix and uterine fibroids were selected as the control group. PCR method was used to detect the expression levels of miR-21, miR-146a and miR-224 in each group. The relationship between clinicopathological characteristics of cervical cancer patients and the expression of miR-21, miR-146a and miR-224 was analyzed. ResultsmiR-224 level in the infection group and the non-infection group was higher than that in the control group, and miR-224 level in the infection group was higher than that in the non-infection group. miR-224 level in the cervical cancer group was higher than those in the control group, LSIL group and HSIL group (P＜0.05). The expression of miR-224 in lesion tissues of cervical cancer patients with low differentiation, patients at FIGO stage Ⅱb－Ⅲa and patients with lymph node metastasis was higher than that in patients with moderate and high differentiation, patients at FIGO stage Ⅰb－Ⅱa, patients without lymph node metastasis and patients without HR-HPV infection (P＜0.05). Differentiation degree, HR-HPV infection, FIGO staging and lymph node metastasis were factors affecting miR-224 level in lesion tissues of patients with cervical lesions. ConclusionThe expression of miR-224 is significantly up-regulated in cervical cancer tissues, and cervical cancer patients with HR-HPV infection have higher miR-224 levels. HR-HPV infection, differentiation degree, FIGO staging and lymph node metastasis are closely related to the expression of miR-224 in cervical cancer tissues. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|