| 李醒,黄俊星.miR-21-5p ceRNA lncRNA MEG3抑制食管鳞癌TE11细胞的增殖和侵袭.[J].中南医学科学杂志.,2024,(4):549-553. |
| miR-21-5p ceRNA lncRNA MEG3抑制食管鳞癌TE11细胞的增殖和侵袭 |
| miR-21-5p ceRNA lncRNA MEG3 inhibits cell proliferation and invasion in TE11 esophageal squamous cell carcinoma |
| 投稿时间：2024-01-09 修订日期：2024-04-08 |
| DOI：10.15972/j.cnki.43-1509/r.2024.04.010 |
| 中文关键词: 食管鳞癌 lncRNA MEG3 ceRNA 增殖 侵袭 |
| 英文关键词:ESCC lncRNA MEG3 ceRNA proliferation invasion |
| 基金项目:泰州市科技项目(TS202001)；泰州市人民医院院级课题项目(ZL202033) |
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| 中文摘要: |
| 目的探讨lncRNA MEG3能否作为miR-21-5p的竞争性内源RNA(ceRNA)抑制食管鳞癌(ESCC)TE11细胞增殖和侵袭。 方法收集食管癌手术患者癌组织及配对的癌旁组织。qRT-PCR法检测lncRNA MEG3和miR-21-5p在配对ESCC组织和癌旁组织中的表达。qRT-PCR法检测lncRNA MEG3和miR-21-5p在各个细胞系中的表达情况。构建过表达MEG3基因的稳定转染细胞株。CCK-8和Transwell小室实验检测lncRNA MEG3对ESCC细胞的增殖和侵袭能力的影响。双荧光素酶检测分析lncRNA MEG3和miR-21-5p是否有靶向关系。采用抗AgO2 RNA结合蛋白免疫沉淀(RIP)实验验证MEG3和miR-21-5p能否在免疫沉淀物中富集。 结果lncRNA MEG3在ESCC组织中的表达低于癌旁组织(P＜0.05)；miR-21-5p在ESCC组织中的表达高于癌旁组织(P＜0.05)；二者的表达呈负相关(P＜0.05)。ESCC患者MEG3的表达与肿瘤大小、分化程度、临床分期、淋巴结转移有关。过表达MEG3可降低miR-21-5p的表达,且MEG3能抑制TE11细胞的增殖和侵袭能力(P＜0.05)。在过表达MEG3的TE11细胞中过表达miR-21-5p,削弱了过表达MEG3对细胞增殖和侵袭的抑制作用(P＜0.05)。双荧光素酶实验结果显示,转染miR-21-5p可降低转染MEG3野生型细胞的荧光素酶相对活性(P＜0.05)。RIP实验证实lncRNA MEG3和miR-21-5p在AgO2免疫沉淀物中显著富集(P＜0.05)。 结论lncRNA MEG3是一种ESCC抑癌基因,作为miR-21-5p的ceRNA抑制了食管鳞癌TE11细胞的增殖和侵袭。 |
| 英文摘要: |
| AimTo detect the expression level of lncRNA MEG3 and miR-21-5p in ESCC tissues and cells, and further explore whether MEG3 could function as a competing endogenous RNA(ceRNA) of miR-21-5p to inhibit ESCC cells proliferation and invasion. MethodsPaired fresh cancer tissues and adjacent tissues were taken from 42 surgical patients with esophageal cancer. The expression of lncRNA MEG3 and miR-21-5p in tissues was examined by quantitative real-time PCR (qRT-PCR). The expression of lncRNA MEG3 and miR-21-5p in each cell line was detected by qRT-PCR. A stable cell line overexpressing MEG3 gene was constructed. CCK-8 and transwell assays were applied respectively to detect the ESCC cell proliferation and invasion. The targeting relationship between lncRNA MEG3 and miR-21-5p was studied by luciferase assay. Anti-AgO2 RNA binding protein immunoprecipitation (RIP) experiment was used to verify whether MEG3 and miR-21-5p can be enriched in the immunoprecipitate. ResultslncRNA MEG3 was decreased in ESCC tissues compared with paracancerous tissues (P＜0.05). On the contrary, miR-21-5p was overexpressed in ESCC tissues compared with paracancerous tissues (P＜0.05). Their expression in ESCC was negatively correlated (P＜0.05). The low expression of MEG3 was positively correlated with large tumor size, worse differentiation, advanced stage and lymph node metastasis. Overexpression of MEG3 can negatively regulate the expression of miR-21-5p (P＜0.05), and can inhibit the proliferation and invasion of TE11 cells (P＜0.05). Overexpression of miR-21-5p in MEG3 overexpression TE11 cells weakened the inhibition of cell proliferation and invasion through the overexpression of MEG3 (P＜0.05). Luciferase assay showed that infection of miR-21-5p could significantly reduce the luciferase activity of cells containing wild-type MEG3 (P＜0.05). RIP experiments showed that lncRNA MEG3 and miR-21-5p were significantly enriched in the immunoprecipitate of AgO2 (P＜0.05). ConclusionlncRNA MEG3 is an ESCC tumor suppressor gene. As a miR-21-5p ceRNA, lncRNA MEG3 inhibits the proliferation and invasion of TE11 ESCC cells. |
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