| 刘禹,江奕,欧意权,赵维超,丁德馨,龙鼎新.纳米聚苯乙烯联合铀暴露对斑马鱼生长发育和运动行为的影响.[J].中南医学科学杂志.,2024,(4):512-516. |
| 纳米聚苯乙烯联合铀暴露对斑马鱼生长发育和运动行为的影响 |
| Effects of polystyrene nanoplastics combined with uranium exposure on growth, development and motor behavior of zebrafish |
| 投稿时间：2024-03-27 修订日期：2024-06-06 |
| DOI：10.15972/j.cnki.43-1509/r.2024.04.003 |
| 中文关键词: 聚苯乙烯 铀 斑马鱼 运动行为 生长发育 |
| 英文关键词:polystyrene uranium zebrafish motor behavior growth and development |
| 基金项目:国家自然科学基金项目(81673227)；湖南省自然科学基金项目(2020JJ4080,2023JJ30497)；湖南省生态环境厅项目(HBKT-2022021)；湖南省科技厅科普专题项目(2022ZK4274)；湖南省大学生创新创业训练计划项目(S202210555300)；湖南省研究生创新基金项目(CX20230994) |
|
| 摘要点击次数: 168 |
| 全文下载次数: 108 |
| 中文摘要: |
| 目的探究纳米聚苯乙烯(PS-NPs)联合铀(U)暴露对斑马鱼生长发育和运动行为的影响。 方法将受精后2 h的斑马鱼健康胚胎随机分为空白对照组(Con组)、U单独暴露组(U组)、PS-NPs单独暴露组(PS-NPs组)、PS-NPs与U联合暴露组(PS-NPs+U组)。记录各组胚胎存活率、孵化率、畸形率。采用Image J测量幼鱼身长、头部面积、卵黄囊面积。采用斑马鱼行为监测仪监测幼鱼白天和夜间行为变化。采用qRT-PCR检测生长发育相关基因(GH、IGF、BMP)的表达水平。 结果与Con组相比,U组胚胎存活率、孵化率均显著降低(P＜0.05),幼鱼畸形率增加并严重发育不良,表现为身长、头部面积减少、卵黄囊面积增加(P＜0.05),U组幼鱼在行为上昼夜都具有较高活性,GH、IGF、BMP表达下调(P＜0.05)；PS-NPs组存活、孵化、发育情况具有与U组相同趋势,但在行为上昼夜都展现出运动能力下降,IGF表达下调(P＜0.05)；PS-NPs+U组畸形率增加(P＜0.05),行为上展现出运动能力下降,BMP表达上调(P＜0.05)。PS-NPs联合U干扰了U单独暴露引起的发育不良,并引起运动能力下降,GH、IGF、BMP表达较U组上调(P＜0.05)。 结论PS-NPs与U联合暴露会引起斑马鱼发育不良和运动能力下降,PS-NPs干扰了U的毒性作用,200 μg/L的PS-NPs和150 μg/L的U属于拮抗毒性。 |
| 英文摘要: |
| AimTo explore the effects of combined exposure of polystyrene nanoplastics (PS-NPs) and uranium (U) on the growth, development and motor behavior of zebrafish. MethodsHealthy embryos 2 hours post-fertilization were randomly divided into blank control group (Con group), U alone exposure group (U group), PS-NPs alone exposure group (PS-NPs group), and PS-NPs and U combined exposure group (PS-NPs+U group). Embryo survival rate, hatching rate and malformation rate of each group were recorded. Body length, head area and yolk sac area of larva were measured by Image J. The zebrafish behavior monitor was used to measure the behavioral changes of larva during the day and night. The expression levels of growth and development-related genes were detected by qRT-PCR. ResultsCompared with Con group, the embryo survival rate and hatching rate of U group were significantly decreased (P＜0.05), and the malformation rate of larva were increased, accompanying with severe dysplasia, reflected as the decrease of body length, head area and increase of yolk sac area (P＜0.05). The larva in U group showed high activity in day and night, as well as decreased expressions of GH, IGF and BMP (P＜0.05). The survival, hatching and development of embryos and larvae in PS-NPs group showed the same trend as those in U group, but the movement ability was decreased day and night, and the expression of IGF was decreased (P＜0.05). The malformation rate of larva in PS-NPs+U group was increased (P＜0.05), the motor behavior was reflected as the decrease of motor ability, and the expression of BMP was increased (P＜0.05). The combination of PS-NPs and U interfered with the dysplasiacaused by U exposure alone, leading to the decline in motor ability. The expression of GH, IGF, and BMP was upregulated compared with the U group (P＜0.05). ConclusionThe combined exposure of PS-NPs and U can cause dysplasia and decreased motor ability of zebrafish. PS-NPs interferes with the toxic effect of U, and 200 μg/L PS-NPs and 150 μg/L U showed antagonistic toxicity. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|