| 黎振东,毛苇.DIAPH3对cagA＋Hp诱导的胃黏膜上皮GES-1细胞EMT及紧密连接蛋白表达的影响.[J].中南医学科学杂志.,2024,(3):364-367, 371. |
| DIAPH3对cagA＋Hp诱导的胃黏膜上皮GES-1细胞EMT及紧密连接蛋白表达的影响 |
| Effect of DIAPH3 on EMT and tight junction protein expression in gastric mucosal epithelial GES-1 cells induced by cagA＋Hp |
| 投稿时间：2023-07-26 修订日期：2023-11-20 |
| DOI：10.15972/j.cnki.43-1509/r.2024.03.011 |
| 中文关键词: 胃癌 Diaphanous相关成蛋白3 幽门螺杆菌 紧密连接蛋白 恶性转化 [ |
| 英文关键词:gastric cancer diaphanous-related formin 3 helicobacter portal gastric mucosal barrier injury malignant transformation |
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| 中文摘要: |
| 目的研究Diaphanous相关成蛋白3(DIAPH3)对细胞毒素相关蛋白A(cagA＋)幽门螺杆菌(Hp)诱导的胃黏膜上皮GES-1细胞上皮间质转化(EMT)及紧密连接蛋白表达的影响。 方法H.pylori J99毒株(cagA＋Hp)与GES-1细胞株共培养,使用DIAPH3干扰片段对细胞进行干预。观察细胞增殖、侵袭、凋亡情况,并检测EMT相关蛋白和紧密连接蛋白变化。 结果显微镜下显示cagA＋Hp共培养的GES-1细胞大小不一,形态发生异变。 与对照组比较,模型组、DIAPH3-NC组和DIAPH3组干预后不同时间的OD570值、侵袭细胞数量均增加,凋亡率降低,N钙黏附蛋白(N-cadherin)、波形蛋白(Vimentin)相对表达量升高,E-钙黏附蛋白(E-cadherin)和紧密连接蛋白(occludin、claudin-1、ZO-1)相对表达量降低(P＜0.05)；与模型组、DIAPH3-NC组比较,DIAPH3组的OD570值、侵袭细胞数量均降低,凋亡率增加,N-cadherin、Vimentin相对表达量降低,E-cadherin、occludin、claudin-1、ZO-1相对表达量升高(P＜0.05)。 结论抑制NIAPH3表达能抑制胃黏膜上皮GES-1细胞的EMT,阻止细胞的侵袭和转移,提高凋亡率,上调紧密连接蛋白表达。 |
| 英文摘要: |
| AimTo investigate the effect of diaphanous-related formin 3 (DIAPH3) on epithelial to mesenchymal transformation (EMT) and tight junction protein expression in gastric epithelial GES-1 cells induced by cytotoxin-related protein (cagA＋) and Helicobacter pylori (Hp). MethodsH.pylori J99 strain (cagA＋Hp) was co-cultured with GES-1 cell line. The cell proliferation, invasion and apoptosis were observed, and the changes of interstitial transition (EMT) related proteins and tight junction proteins were detected. ResultsCompared with the control group, the OD570 value and the number of invasive cells in the model, DIAPH3-NC, and DIAPH3 groups increased at different times after intervention, and apoptosis rate decreased. The relative expressions of N-cadherin and Vimentin increased. The relative expression of E-cadherin and tight junction proteins (E-Cadherin, claudin-1, ZO-1) decreased (P＜0.05);Compared with the model group and the DIAPH3-NC group, the OD570 value, the number of invasive cells, apoptosis rate, and the relative expression levels of N-cadherin and Vimentin decreased in the DIAPH3 group. The relative expression of E-cadherin, occludin, claudin-1 and ZO-1 increased (P＜0.05). ConclusionInhibition of NIAPH3 expression can inhibit EMT in gastric epithelium GES-1 cells, prevent cell invasion and metastasis, increase apoptosis rate, and up-regulate the expression of tight junction protein. |
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