| 赵威瑾,卫雷,李畅,李知娟,王经.川芎多糖通过AMPK/Nrf2信号通路减轻大鼠心肌缺血再灌注损伤.[J].中南医学科学杂志.,2024,(3):348-352. |
| 川芎多糖通过AMPK/Nrf2信号通路减轻大鼠心肌缺血再灌注损伤 |
| Ligusticum chuanxiong polysaccharide alleviates myocardial ischemia reperfusion injury in rats via AMPK/Nrf2 signaling pathway |
| 投稿时间：2023-04-10 修订日期：2024-03-22 |
| DOI：10.15972/j.cnki.43-1509/r.2024.03.008 |
| 中文关键词: 心肌缺血再灌注 川芎多糖 AMPK/Nrf2信号通路 氧化应激 大鼠 [ |
| 英文关键词:MIRI LCPS AMPK/Nrf2 signaling pathway oxidative stress rats |
| 基金项目:河北省医学科学研究课题(20220526) |
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| 中文摘要: |
| 目的探讨川芎多糖(LCPS)对大鼠心肌缺血再灌注损伤(MIRI)及腺苷酸活化蛋白激酶(AMPK)/核因子红系2相关因子2(Nrf2)信号通路的影响。 方法将120只SD大鼠随机均分为假手术组、模型组、LCPS组、AMPK激动剂AICAR组和LCPS+AICAR组。除假手术组外,其他各组构建MIRI大鼠模型。超声影像系统检测各组心功能指标,ELISA法检测血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)、心肌肌钙蛋白(cTnI)含量,计算心脏指数,TTC染色法检测心肌梗死面积,HE染色法观察心肌组织病理学改变,分光光度法检测丙二醛(MDA)和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,Western blotting法检测心肌组织AMPK、磷酸化(p)-AMPK、Nrf2、血红素加氧酶-1(HO-1)蛋白表达。 结果LCPS能够明显改善MIRI大鼠心功能及心肌组织病变,减少心肌梗死面积,降低MDA、心脏指数以及血清LDH、CK-MB、cTnI水平,升高SOD、GSH-Px活性、p-AMPK/AMPK比值以及Nrf2、HO-1表达水平。LCPS联合AICAR对各指标的影响优于LCPS组和AICAR组(P＜0.05)。 结论LCPS具有减轻大鼠MIRI的作用,其机制可能与激活AMPK/Nrf2信号通路、抑制氧化应激有关。 |
| 英文摘要: |
| AimTo investigate the effects of ligusticum chuanxiong polysaccharide (LCPS) on myocardial ischemia reperfusion injury (MIRI) and the roles of adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-erythroid 2-related factor 2 (Nrf2) signaling pathway in rats. Methods120 SD rats were randomly divided into sham operation group, model group, LCPS group, AMPK activator AICAR group and LCPS+AICAR group. Except for the sham surgery group, other groups were used to construct MIRI rat models. The cardiac function indexes were detected by echocardiography, the content of creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), myocardial troponin (cTnI) in serum were determined by ELISA, the cardiac index was alculated; the myocardial infarction size was detected by TTC staining, the myocardial histopathological changes were observed by HE staining, the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) were determined by spectrophotometry, and the protein expression of AMPK, phosphorylation (p)-AMPK, Nrf2, heme oxygenase-1 (HO-1) were detected by Western blotting. ResultsLCPS can significantly improve cardiac function and myocardial tissue damage in MIRI rats, reduce myocardial infarction area, MDA levels, cardiac index, serum LDH, CK-MB, cTnI levels, increase SOD, GSH-Px activity, p-AMPK/AMPK ratio, and Nrf2 and HO-1 expression levels. The impact of LCPS combined with AICAR on various indicators is larger than that of the LCPS group and AICAR group (P＜0.05). ConclusionLCPS can reduce the MIRI in rats, and the mechanism may be related to the activation of AMPK/Nrf2 signaling pathway and the inhibition of oxidative stress. |
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