| 曾茹,毛山山.miR-506对急性胰腺炎大鼠巨噬细胞迁移抑制因子及肠屏障功能影响.[J].中南医学科学杂志.,2024,(3):344-347. |
| miR-506对急性胰腺炎大鼠巨噬细胞迁移抑制因子及肠屏障功能影响 |
| Effects of miR-506 on macrophage migration inhibitory factor and intestinal barrier function in rats with acute pancreatitis |
| 投稿时间：2023-05-25 修订日期：2023-11-18 |
| DOI：10.15972/j.cnki.43-1509/r.2024.03.007 |
| 中文关键词: 急性胰腺炎 miR-506 巨噬细胞迁移抑制因子 肠屏障功能 [ |
| 英文关键词:acute pancreatitis miR-506 macrophage migration inhibitor factor intestinal barrier function |
| 基金项目:海南省自然科学基金高层次人才项目(822RC862) |
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| 中文摘要: |
| 目的探讨微小RNA-506(miR-506)对急性胰腺炎大鼠(AP)巨噬细胞迁移抑制因子(MIF)及肠屏障功能的影响。 方法将SPF级SD大鼠50只随机均分为对照组、模型组、miR-506组、MIF组、miR-506＋MIF组。观察各组胰腺组织病理学变化、观察胰腺组织病理学变化、肠屏障功能[血清淀粉酶、内毒素、血浆D-乳酸、乳果糖/甘露醇排除率(L/M)、细菌移位率]及MIF水平变化。。 结果HE染色显示,对照组胰腺组织形态结构正常,无明显的病理改变；模型组、MIF组、miR-506组及miR-506+MIF组均有不同程度胰腺坏死,MIF组病变最为严重；miR-506组病变最轻。miR-506组胰腺病理评分、血清淀粉酶、内毒素、MIF、血浆D-乳酸水平、L/M、细菌移位率及MIF mRNA相对表达量较其他干预组降低,miR-506相对表达量则升高(P＜0.05)；miR-506+MIF组血清淀粉酶、内毒素、MIF、血浆D-乳酸水平、L/M、细菌移位率及MIF mRNA相对表达量较miR-506组升高,miR-506相对表达量较miR-506组下降(P＜0.05)。 结论miR-506的高表达减轻AP大鼠病变,改善肠屏障功能,其作用可能与抑制MIF有关。 |
| 英文摘要: |
| AimTo investigate the effects of microRNA-506 (miR-506) on macrophage migration inhibitory factor (MIF) and intestinal barrier function in acute pancreatitis (AP) rats. MethodsFifty SPF grade SD rats were selected to establish AP model and treated with miR-506 and MIF. Pancreatic histopathological changes, intestinal barrier function [serum amylase, endotoxin, plasma D-lactic acid, lactulose/mannitol exclusion rate (L/M), bacterial translocation rate] and MIF level were observed. ResultsHE staining showed that the control group had normal pancreatic tissue morphology and structure, with no obvious pathological changes. Model group, MIF group, miR-506 group and miR-506+MIF group all had different degrees of pancreatic necrosis, and the lesions in MIF group were the most serious. miR-506 group had the mildest pathological changes. The pancreatic pathological score, serum amylase, endotoxin, MIF, plasma D-mammary gland level, lactulose/mannitol exclusion rate (L/M), bacterial translocation rate and MIF mRNA relative expression level of miR-506 group were lower than those of other groups, while the relative expression level of miR-506 was increased (P＜0.05).After dual intervention with miR-506 and MIF, serum amylase, endotoxin, MIF, plasma D-mammary gland level, L/M, bacterial migration rate and relative expression level of MIF mRNA in miR-506+MIF group were higher than those in miR-506 group, and the relative expression level of miR-506 was lower than that in miR-506 group (P＜0.05). ConclusionThe high expression of miR-506 alleviates the lesions of AP rats and improves the intestinal barrier function, which may be related to the inhibition of MIF. |
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